Molecular phenomics of a high-calorie diet-induced porcine model of prepubertal obesity
Autor: | J. Tibau, José C. E. Serrano, Joaquim Puigjaner, Reinald Pamplona, M Rodrı́guez-Palmero, Lluís Arola, Manuel Portero-Otin, Joaquim Sol, Anna Castell, Maria Font-i-Furnols, Reyna Rodriguez-Mortera, Rebeca Berdún, Joan Prat, Meritxell Martín-Gari, Anna Crescenti, Raquel Quintanilla, Josep Manuel Fernández-Real, Mariona Jové, Mònica Sabater, E. Fàbrega, Helene Rassendren, Francisco J. Ortega, Anna Cassanyé, Jose A. Moreno |
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Přispěvatelé: | Producció Animal, Genètica i Millora Animal, Benestar Animal, Qualitat i Tecnologia Alimentària |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Pediatric Obesity Calorie Endocrinology Diabetes and Metabolism Clinical Biochemistry Adipose tissue 030209 endocrinology & metabolism Preclinical models Biology Diet High-Fat Biochemistry 03 medical and health sciences Eating 0302 clinical medicine Insulin resistance Internal medicine Lipidomics medicine Animals Humans Metabolomics Phenomics Molecular Biology Triglycerides Adiposity Nutrition and Dietetics Puberty Lipidome medicine.disease Obesity Monoacylglycerol lipase Disease Models Animal 030104 developmental biology Endocrinology Leukocytes Mononuclear Female medicine.symptom Weight gain Biomarkers |
Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname Repositorio Abierto de la UdL Universitad de Lleida Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) |
Popis: | As obesity incidence is alarmingly rising among young individuals, we aimed to characterize an experimental model of this situation, considering the similarity between human and porcine physiology. For this reason, we fed prepubertal (63 days old) Duroc breed females (n=21) either with a standard growth diet (3800 kcal/day) or one with a high-calorie content (5200 kcal/day) during 70 days. Computerized tomography, mass-spectrometry-based metabolomics and lipidomics, as well as peripheral blood mononuclear cell transcriptomics, were applied to define traits linked to high-calorie intake. Samples from a human cohort confirmed potential lipidomic markers. Compared to those fed a standard growth diet, pigs fed a high-calorie diet showed an increased weight gain (13%), much higher adiposity (53%), hypertriacylglyceridemia and hypercholesterolemia in parallel to insulin resistance. This diet induced marked changes in the circulating lipidome, particularly in phosphatidylethanolamine-type molecules. Also, circulating specific diacylglycerol and monoacylglycerol contents correlated with visceral fat and intrahepatic triacylglycerol concentrations. Specific lipids associated with obesity in swine (mainly belonging to glycerophospholipid, triacylglyceride and sterol classes) were also linked with obesity traits in the human cohort, reinforcing the usefulness of the chosen approach. Interestingly, no overt inflammation in plasma or adipose tissue was evident in this model. The presented model is useful as a preclinical surrogate of prepubertal obesity in order to ascertain the pathophysiology interactions between energy intake and obesity development. Supported by Centro para el Desarrollo Tecnológico e Industrial, Spain, Project reference: IPT-20111008, and Generalitat de Catalunya grants 2017SGR1719 and 2017SGR696. MJ is a "Serra Hunter" program fellow. Supported by Instituto de Salud Carlos III, Spain, Project reference: 17-00134, co-financed by FEDER Funds A way to make Europe. |
Databáze: | OpenAIRE |
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