Molecular phenomics of a high-calorie diet-induced porcine model of prepubertal obesity

Autor: J. Tibau, José C. E. Serrano, Joaquim Puigjaner, Reinald Pamplona, M Rodrı́guez-Palmero, Lluís Arola, Manuel Portero-Otin, Joaquim Sol, Anna Castell, Maria Font-i-Furnols, Reyna Rodriguez-Mortera, Rebeca Berdún, Joan Prat, Meritxell Martín-Gari, Anna Crescenti, Raquel Quintanilla, Josep Manuel Fernández-Real, Mariona Jové, Mònica Sabater, E. Fàbrega, Helene Rassendren, Francisco J. Ortega, Anna Cassanyé, Jose A. Moreno
Přispěvatelé: Producció Animal, Genètica i Millora Animal, Benestar Animal, Qualitat i Tecnologia Alimentària
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Recercat. Dipósit de la Recerca de Catalunya
instname
Repositorio Abierto de la UdL
Universitad de Lleida
Recercat: Dipósit de la Recerca de Catalunya
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
IRTA Pubpro. Open Digital Archive
Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Popis: As obesity incidence is alarmingly rising among young individuals, we aimed to characterize an experimental model of this situation, considering the similarity between human and porcine physiology. For this reason, we fed prepubertal (63 days old) Duroc breed females (n=21) either with a standard growth diet (3800 kcal/day) or one with a high-calorie content (5200 kcal/day) during 70 days. Computerized tomography, mass-spectrometry-based metabolomics and lipidomics, as well as peripheral blood mononuclear cell transcriptomics, were applied to define traits linked to high-calorie intake. Samples from a human cohort confirmed potential lipidomic markers. Compared to those fed a standard growth diet, pigs fed a high-calorie diet showed an increased weight gain (13%), much higher adiposity (53%), hypertriacylglyceridemia and hypercholesterolemia in parallel to insulin resistance. This diet induced marked changes in the circulating lipidome, particularly in phosphatidylethanolamine-type molecules. Also, circulating specific diacylglycerol and monoacylglycerol contents correlated with visceral fat and intrahepatic triacylglycerol concentrations. Specific lipids associated with obesity in swine (mainly belonging to glycerophospholipid, triacylglyceride and sterol classes) were also linked with obesity traits in the human cohort, reinforcing the usefulness of the chosen approach. Interestingly, no overt inflammation in plasma or adipose tissue was evident in this model. The presented model is useful as a preclinical surrogate of prepubertal obesity in order to ascertain the pathophysiology interactions between energy intake and obesity development. Supported by Centro para el Desarrollo Tecnológico e Industrial, Spain, Project reference: IPT-20111008, and Generalitat de Catalunya grants 2017SGR1719 and 2017SGR696. MJ is a "Serra Hunter" program fellow. Supported by Instituto de Salud Carlos III, Spain, Project reference: 17-00134, co-financed by FEDER Funds A way to make Europe.
Databáze: OpenAIRE