Evidence for Somatic Generation of Antibody Diversity
| Autor: | C. Steinberg, A. Bernardini, Susumu Tonegawa, S. Dube |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Immunoglobulins
Biology Homology (biology) Cell Line Iodine Radioisotopes Immunoglobulin kappa-Chains Mice Nucleic acid thermodynamics chemistry.chemical_compound Animals Amino Acid Sequence RNA Messenger Immunoglobulin Fragments Gene Mice Inbred BALB C Messenger RNA Multidisciplinary Base Sequence Nucleic Acid Hybridization RNA Antibody Diversity DNA Genetic code Molecular biology Kinetics Genes chemistry Genetic Code Autoradiography Electrophoresis Polyacrylamide Gel Female Biological Sciences: Genetics Plasmacytoma |
| Zdroj: | Proceedings of the National Academy of Sciences. 71:4027-4031 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | RNA preparations containing 70-80% mouse κ-chain mRNA have been prepared. The remainder consists of many RNA species, each of which represents a small fraction of the total RNA. The κ-chain mRNA preparation hybridizes with mouse liver DNA with bi-phasic kinetics, indicating that it consists of two fractions —“unique” and “reiterated.” Competition hybridization experiments show that the homology among the unique fractions from different mRNAs is the same as the homology among the amino acid sequences of the corresponding κ-chains. Hence, in addition to the C-region (constant-region) sequences, (most of) the V-region (variable-region) sequences are also derived from unique germ line genes. The reiterated fractions from different κ-chain mRNAs show essentially complete homology with each other. This fraction seems to consist mostly of sequences which do not code for amino-acid sequences of the secreted polypeptide chain, i.e., the “external” section of the mRNA molecule. It is concluded that the number of germ line genes is too small to account for the observed diversity of antibody molecules. |
| Databáze: | OpenAIRE |
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