The cultivation of human retinal pigment epithelial cells on Bombyx mori silk fibroin
| Autor: | Anthony S. L. Kwan, Traian V. Chirila, Karina A. George, Audra M. A. Shadforth, Damien G. Harkin |
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| Rok vydání: | 2012 |
| Předmět: |
Materials science
Cell Transplantation 111301 Ophthalmology Silk Biophysics Silk fibroin Fibroin Bioengineering Retinal Pigment Epithelium Bruch's membrane Biomaterials chemistry.chemical_compound Tissue culture Bombyx mori Polymer chemistry medicine Animals Humans Cells Cultured Transplantation Extracellular Matrix Proteins Retinal pigment epithelium biology Age-related macular degeneration Membranes Artificial Retinal Bombyx biology.organism_classification Immunohistochemistry Molecular biology Blood Membrane medicine.anatomical_structure chemistry Mechanics of Materials 090301 Biomaterials Ceramics and Composites Fibroins |
| Zdroj: | Biomaterials |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2012.02.040 |
| Popis: | We have presently evaluated membranes prepared from Bombyx mori silk fibroin (BMSF), for their potential use as a prosthetic Bruch's membrane and carrier substrate for human retinal pigment epithelial (RPE) cell transplantation. Porous BMSF membranes measuring 3 μm in thickness were prepared from aqueous solutions (3% w/v) containing poly(ethylene oxide) (0.09%). The permeability coefficient for membranes was between 3 and 9 × 10(-5) cm/s by using Allura red or 70 kDa FITC-dextran respectively. Average pore size (±sd) was 4.9 ± 2.3 μm and 2.9 ± 1.5 μm for upper and lower membrane surfaces respectively. Optimal attachment of ARPE-19 cells to BMSF membrane was achieved by pre-coating with vitronectin (1 μg/mL). ARPE-19 cultures maintained in low serum on BMSF membranes for approximately 8 weeks, developed a cobble-stoned morphology accompanied by a cortical distribution of F-actin and ZO-1. Similar results were obtained using primary cultures of human RPE cells, but cultures took noticeably longer to establish on BMSF compared with tissue culture plastic. These findings encourage further studies of BMSF as a substrate for RPE cell transplantation. |
| Databáze: | OpenAIRE |
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