Production of a cytotoxic human monoclonal antibody with specificity for HLA-DR4 and -DRw10 by cells derived from a highly sensitized kidney recipient
| Autor: | Benjamin A. Bradley, Linda Barber, Robert I. Lechler, K. Yousaf, P.D. Bowerman, R. J. T. Hancock, J. E. Yendle, B.B. Cohen, I.M. Roberts |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cytotoxicity
Immunologic musculoskeletal diseases medicine.drug_class Blotting Western Immunology chemical and pharmacologic phenomena Cell Separation Transfection Immunoglobulin E Monoclonal antibody Cell Line Mice Antibody Specificity immune system diseases HLA-DR4 Antigen medicine Animals Humans Immunology and Allergy Cytotoxic T cell skin and connective tissue diseases Cytotoxicity B-Lymphocytes Hybridomas biology medicine.diagnostic_test Antibodies Monoclonal HLA-DR Antigens General Medicine Flow Cytometry Kidney Transplantation Virology Molecular biology Transplantation Cell culture biology.protein Antibody Tissue typing Polymorphism Restriction Fragment Length |
| Zdroj: | Human Immunology. 27:167-181 |
| ISSN: | 0198-8859 |
| DOI: | 10.1016/0198-8859(90)90048-t |
| Popis: | A human monoclonal antibody which reacts preferentially with HLA-DR4 and -DRw10 B-cell targets has been produced. A human B-cell line, secreting antibody which reacted preferentially with DR4 and DR1 targets, was derived from a highly sensitized kidney recipient who had rejected two grafts. This line was fused with the mouse myeloma P3X63Ag8.653 and a selected hybridoma cloned. The clones secrete IgM(λ), which reacts strongly with HLA-DR4 and -DRw10 and more weakly with -DRw14 and a proportion of -DR1 B cells in cytotoxicity assays. Using B-cell lines as targets in cytotoxicity and enzyme-linked immunosorbent assays, the antibody gives a broader pattern of reaction, reacting with HLA-DR1, -DR4, -DR9, -DRw10, -DRw14, and some -DR2 targets. The antibody (NI) is currently in use as a reagent for tissue typing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|