Thermal profile with alternately raised and lowered annealing temperature improves the PCR amplification using highly degenerate primers
| Autor: | Paweł Sachadyn, Gabriela Sobiewska, Józef Kur |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Annealing (metallurgy)
Chemistry Touchdown polymerase chain reaction Pcr cloning Analytical chemistry Temperature Molecular biology Polymerase Chain Reaction Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology law.invention Glucosamine-6-Phosphate Synthase law Degenerate primer Thermal Polymerase chain reaction DNA Primers |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0001-527X |
| Popis: | The proposed here PCR thermal profile improves the specificity and efficiency of PCR using highly degenerate primers, especially in the case of larger PCR products (around 2000 bp and more). The improvement is achieved by the use of a specific annealing temperature in the beginning cycles and the alternate lowering and raising of the annealing temperature in the subsequent cycles. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|