Cytochrome oxidase gene sequencing reveals channel catfish ovary cell line is contaminated with brown bullhead cells
| Autor: | Larry A. Hanson, Lorelei Ford, Thomas B. Waltzek, Kuttichantran Subramaniam, Paul R. Bowser |
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| Rok vydání: | 2020 |
| Předmět: |
Fish Proteins
0301 basic medicine animal structures Veterinary (miscellaneous) Ovary macromolecular substances Aquatic Science Biology DNA sequencing Cell Line Electron Transport Complex IV 03 medical and health sciences medicine Animals Cytochrome c oxidase fungi Sequence Analysis DNA 04 agricultural and veterinary sciences Ameiurus biology.organism_classification Molecular biology Ictaluridae 030104 developmental biology medicine.anatomical_structure Cell culture Ictalurus 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Female Ovary cell Catfish |
| Zdroj: | Journal of Fish Diseases. 44:119-122 |
| ISSN: | 1365-2761 0140-7775 |
| DOI: | 10.1111/jfd.13278 |
| Popis: | The channel catfish (Ictalurus punctatus, Rafinesque) ovary (CCO) cell line is the standard cell line used for channel catfish diagnostics. Next-gen sequencing studies of a virus cultured in the CCO cells revealed mitochondrial sequences matching those of brown bullhead (Ameiurus nebulosus, Lesueur). Therefore, we systematically performed partial cytochrome oxidase 1 gene sequencing of several sources of the CCO cell line and all matched the brown bullhead and not the channel catfish. |
| Databáze: | OpenAIRE |
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