Protein-resistant properties of poly(N-vinylpyrrolidone)-modified gold surfaces: The advantage of bottle-brushes over linear brushes
| Autor: | Shuxiang Zhang, Hong Chen, Yishi Dong, Wenying Liu, Peixi Wang, Zhaoqiang Wu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Biocompatibility Polymers Surface Properties Glycopolymer macromolecular substances 02 engineering and technology 01 natural sciences Contact angle chemistry.chemical_compound Colloid and Surface Chemistry X-ray photoelectron spectroscopy 0103 physical sciences Particle Size Physical and Theoretical Chemistry Fourier transform infrared spectroscopy Molecular Structure 010304 chemical physics technology industry and agriculture Fibrinogen Serum Albumin Bovine Surfaces and Interfaces General Medicine Quartz crystal microbalance 021001 nanoscience & nanotechnology Pyrrolidinones Polymerization chemistry Chemical engineering Quartz Crystal Microbalance Techniques Muramidase Adsorption Gold 0210 nano-technology Biosensor Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 177:448-453 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2019.02.030 |
| Popis: | Poly(N-vinylpyrrolidone) (PVP)-modified surfaces have been shown to possess excellent protein resistance and good biocompatibility. However, PVP-modified surfaces with different molecular architectures have not been prepared, and their protein-resistant properties have not been studied. Herein, gold surfaces modified with linear PVP brush and PVP bottle-brush architectures were prepared by photoinitiated surface grafting polymerization. Ellipsometry, X-ray photoelectron spectroscopy (XPS), water contact angle, Fourier transform infrared (FTIR) spectroscopy and atomic force microscopy (AFM) were utilized to characterize the prepared surfaces. The protein-resistant properties were investigated by a quartz crystal microbalance with dissipation (QCM-D) with bovine serum albumin (BSA), fibrinogen (Fg) and lysozyme (Lyz). Compared with the ungrafted QCM-D chips, the PVP bottle-brush-grafted chips (9.3 nm thickness) showed superior protein resistance over linear PVP brush-grafted chips (9.9 nm thickness). Furthermore, the PVP bottle-brushes reduced the levels of BSA, Fg and Lyz adsorption by 97%, 85% and 69%, respectively. Moreover, to demonstrate potential applications as functional biosensors and in the biomedical field, PVP bottle-brushes containing glycopolymer-grafted gold surfaces were fabricated. Laser scanning confocal microscopy (LSCM) demonstrated that these glycopolymer surfaces showed excellent protein resistance and specific ConA binding ability. Overall, we speculate that the data presented here can provide useful information for the development of excellent antifouling materials and functional biosensors. |
| Databáze: | OpenAIRE |
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