Cellular alterations and modulation of protein expression in bitumen-challenged human osteoblast cells
| Autor: | Kannan Krishnamurthi, Subin T. Surendran, Pravin K. Naoghare, Alka Dhondge, Tapan Chakrabarti, Muhil Vannan Seralathan, Sivanesan Saravana Devi |
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| Rok vydání: | 2012 |
| Předmět: |
Proteome
Health Toxicology and Mutagenesis Cell Gene Expression Mice Nude Biology Hazardous Substances Mice Downregulation and upregulation In vivo medicine Animals Humans Environmental Chemistry Cell adhesion Carcinogen Severe combined immunodeficiency Osteoblasts General Medicine medicine.disease Pollution Molecular biology Hydrocarbons In vitro Cell Transformation Neoplastic medicine.anatomical_structure Immunology Cancer cell Carcinogens Female |
| Zdroj: | Environmental Science and Pollution Research. 19:4030-4041 |
| ISSN: | 1614-7499 0944-1344 |
| Popis: | There are many arguments on the carcinogenic potential of bitumen extract. The mechanism of bitumen-induced damage is not well understood at the molecular level. Therefore, in the present study, cell-transforming and tumor-inducing potential of bitumen extract was studied using in vitro [human osteosarcoma (HOS) cells] and in vivo [nude and severe combined immunodeficiency (SCID) mice] models. Gas chromatography/mass spectrometry (GC/MS) analysis was carried out to find out the existence of carcinogenic compounds in the bitumen extract. Cell transformation test, anchorage independence assay, karyotyping assay, tumorigenicity assay, and 2-DE analysis were used to find out the effect of bitumen using the in vitro and in vivo models. GC/MS analysis showed the existence of carcinogenic compounds in the bitumen extract. HOS cells were treated with different concentrations (25, 50, and 100 μl/ml) of bitumen extract. Compared to the parental HOS cells, bitumen transformants (HOS T1 and HOS T2) showed the characteristics of anchorage independency, chromosomal anomaly, and cellular transformation. Interestingly, bitumen transformants were not able to form tumor in nude/SCID mice. Proteomic analysis revealed the existence of 19 differentially expressed proteins involved in progression of cancer, angiogenesis, cell adhesion, etc. Exposure of bitumen extract to HOS cells results in the cellular transformation similar to cancer cells and can modulate proteins involved in the progression of cancer. We state that the non-tumorogenic potential of bitumen transformant in nude/SCID mice can be attributed to the downregulation of galectin-1, chromodomain helicase DNA-binding protein 1-like gene, and membrane-associated guanylate kinase 2 protein. |
| Databáze: | OpenAIRE |
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