HPLC-CUPRAC post-column derivatization method for the determination of antioxidants: a performance comparison between porous silica and core-shell column packing
| Autor: | Socrates Jose P. Canete, Syed A. Haque |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
lcsh:Analytical chemistry
General Physics and Astronomy 02 engineering and technology Epigallocatechin gallate Porous silica column 01 natural sciences High-performance liquid chromatography Post-column derivatization General Biochemistry Genetics and Molecular Biology Antioxidants lcsh:Chemistry chemistry.chemical_compound General Materials Science Derivatization Porosity Column (data store) Core-shell column General Environmental Science CUPRAC Chromatography lcsh:QD71-142 010401 analytical chemistry food and beverages Catechin General Chemistry 021001 nanoscience & nanotechnology Post column derivatization 0104 chemical sciences chemistry lcsh:QD1-999 Reagent 0210 nano-technology Research Article |
| Zdroj: | Journal of Analytical Science and Technology, Vol 9, Iss 1, Pp 1-9 (2018) Journal of Analytical Science and Technology |
| ISSN: | 2093-3371 |
| DOI: | 10.1186/s40543-018-0137-1 |
| Popis: | Background An HPLC method employing a post-column derivatization strategy using the cupric reducing antioxidant capacity reagent (CUPRAC reagent) for the determining antioxidants in plant-based materials leverages the separation capability of regular HPLC approaches while allowing for detection specificity for antioxidants. Methods Three different column types, namely core-shell and porous silica including two chemically different core-shell materials (namely phenyl-hexyl and C18), were evaluated to assess potential improvements that could be attained by changing from a porous silica matrix to a core-shell matrix. Tea extracts were used as sample matrices for the evaluation specifically looking at catechin and epigallocatechin gallate (EGCG). Results Both the C18 and phenyl-hexyl core-shell columns showed better performance compared to the C18 porous silica one in terms of separation, peak shape, and retention time. Among the two core-shell materials, the phenyl-hexyl column showed better resolving power compared to the C18 column. Conclusions The CUPRAC post-column derivatization method can be improved using core-shell columns and suitable for quantifying antioxidants, exemplified by catechin and EGCG, in tea samples. |
| Databáze: | OpenAIRE |
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