Comparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A02
| Autor: | Kaiwei Si, Bai Xuelian, Shi Lingling, Ting Liu, Dewen Liu, Yong Liang, Hong Zhang, Yuesen Yue, Dan Dong, Taotao Zhang, Liu Weicheng, Huiling Wu, Zhaofeng Tian, Juan Zhao |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genome evolution Natamycin Science 030106 microbiology Sequence assembly Secondary Metabolism Genomics Computational biology Genome Streptomyces Article Microbiology 03 medical and health sciences Gene Expression Regulation Fungal RNA Ribosomal 16S Gene Phylogeny Comparative genomics Whole genome sequencing Multidisciplinary biology Computational Biology biology.organism_classification 030104 developmental biology Multigene Family Medicine Genome Bacterial Signal Transduction |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
| ISSN: | 2045-2322 |
| Popis: | Streptomyces lydicus A02 is used by industry because it has a higher natamycin-producing capacity than the reference strain S. natalensis ATCC 27448. We sequenced the complete genome of A02 using next-generation sequencing platforms, and to achieve better sequence coverage and genome assembly, we utilized single-molecule real-time (SMRT) sequencing. The assembled genome comprises a 9,307,519-bp linear chromosome with a GC content of 70.67%, and contained 8,888 predicted genes. Comparative genomics and natamycin biosynthetic gene cluster (BGC) analysis showed that BGC are highly conserved among evolutionarily diverse strains, and they also shared closer genome evolution compared with other Streptomyces species. Forty gene clusters were predicted to involve in the secondary metabolism of A02, and it was richly displayed in two-component signal transduction systems (TCS) in the genome, indicating a complex regulatory systems and high diversity of metabolites. Disruption of the phoP gene of the phoR-phoP TCS and nsdA gene confirmed phosphate sensitivity and global negative regulation of natamycin production. The genome sequence and analyses presented in this study provide an important molecular basis for research on natamycin production in Streptomyces, which could facilitate rational genome modification to improve the industrial use of A02. |
| Databáze: | OpenAIRE |
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