Molecularly imprinted polymers with novel functional monomer for selective solid-phase extraction of gastrodin from the aqueous extract of Gastrodia elata
| Autor: | Gao Qianshan, Yanling Geng, Luqi Huang, Wenhua Ji, Xiao Wang, Xiuli Ma, Chen Lingxiao |
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| Rok vydání: | 2014 |
| Předmět: |
Polymers
Scanning electron microscope Plant Roots Biochemistry Polymerization Styrenes Analytical Chemistry Molecular Imprinting chemistry.chemical_compound Glucosides Solid phase extraction Gastrodin Benzyl Alcohols chemistry.chemical_classification Gastrodia Chromatography biology Plant Extracts Solid Phase Extraction Organic Chemistry Molecularly imprinted polymer Water Hydrogen Bonding General Medicine Polymer biology.organism_classification Gastrodia elata Allyl Compounds Solvent Functional monomer chemistry Solvents |
| Zdroj: | Journal of Chromatography A. 1342:1-7 |
| ISSN: | 0021-9673 |
| Popis: | Molecular imprinted polymers (MIPs) with high selectivity and affinity to gastrodin in water were designed using allyl 2,3,4,6-tetra-O-acetyl-glucopyranoside (TAGL) and 1,2,3,4,5-pentafluoro-6-vinylbenzene (PFVB) as novel functional monomers. Binding characterization of pre-polymerization complexes was researched by nuclear magnetic resonance (NMR) and the MIPs were characterized by scanning electron microscopy (SEM). The properties involving adsorption isotherm, adsorption kinetics and selective recognition capacity were evaluated. The MIPs/TAGL exhibited good site accessibility in which it only took 30min to achieve adsorption equilibrium and highly selective recognition for the template. Furthermore, the performance of the MIPs/TAGL as solid phase extraction material was investigated in detail and hot water at 50°C served as the eluting solvent. Pure gastrodin with the recovery of 76.6% was obtained from the aqueous extract of Gastrodia elata roots. |
| Databáze: | OpenAIRE |
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