Characterization of DBT Cell Clones Derived from Cells Persistently Infected with the JHM Strain of Mouse Hepatitis Virus
| Autor: | Tetsuya Mizutani, Tomomasa Watanabe, Kozue Ishida, Shigeo Namioka, Masanobu Hayashi, Akihiko Maeda |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Viral protein
viruses Molecular Sequence Data Cell Viral Plaque Assay Astrocytoma medicine.disease_cause Polymerase Chain Reaction Virus Microbiology Rodent Diseases Mice Mouse hepatitis virus Cell Clone Tumor Cells Cultured medicine Animals DNA Primers Brain Chemistry Murine hepatitis virus Mutation Base Sequence General Veterinary biology Brain Neoplasms virus diseases RNA biology.organism_classification Virology Clone Cells medicine.anatomical_structure Superinfection DNA Viral RNA Viral Coronavirus Infections |
| Zdroj: | Journal of Veterinary Medical Science. 57:813-817 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.57.813 |
| Popis: | Twelve clones derived from the cells persistently infected with the JHM strain (JHMV) of mouse hepatitis virus (MHV) were established from mouse astrocytoma-derived DBT cells and characterized. All the cell clones were resistant to superinfection with MHV. Only one of the persistently infected cell clone synthesized viral RNA and proteins and produced virus particles. Viral RNA was detectable in some other cell clones without production of viral protein nor the virus. No cell clones exhibited contact fusion activity. The results suggested that such variety of cell clones might have resulted from persistent infection with JHMV. |
| Databáze: | OpenAIRE |
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