Environmental influence on age-related changes of human lymphocyte membrane viscosity using severe combined immunodeficiency mice as an in vivo model
| Autor: | Günther Böck, Dieter Schönitzer, Georg Wick, Hermann Dietrich, Günther Jürgens, Christian Maczek |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Aging medicine.medical_specialty Cell Transplantation Membrane Fluidity Lipoproteins T-Lymphocytes Membrane lipids Lymphocyte Mice SCID Lymphocyte proliferation Biology Binding Competitive Biochemistry Mice Endocrinology In vivo Internal medicine Genetics medicine Animals Humans Lovastatin Lymphocytes Molecular Biology Aged Mice Inbred BALB C Severe combined immunodeficiency Anticholesteremic Agents Cell Membrane Lipid metabolism Cell Biology medicine.disease Lymphocyte Subsets In vitro Lipoproteins LDL Membrane medicine.anatomical_structure Receptors LDL Immunology Leukocytes Mononuclear Leukocyte Common Antigens Female |
| Zdroj: | Experimental Gerontology. 33:485-498 |
| ISSN: | 0531-5565 |
| Popis: | Peripheral blood lymphocytes (PBL) of healthy elderly people show increased plasma membrane viscosity compared to young subjects, that inversely correlates with lymphocyte proliferation after mitogen stimulation in vitro. Maintenance of a constant membrane viscosity, which is necessary for proper cell function, is crucially dependent on the membrane lipid composition. The cellular lipid metabolism, and thus lymphocyte function, may be subject to modulation by diet or drugs. To study the susceptibility of membrane viscosity to environmental conditions, we established an in vivo model using severe combined immunodeficiency (SCID) mice: human peripheral blood lymphocytes from healthy young and old subjects were engrafted for three days intraperitoneally into SCID mice to offer identical environmental conditions. First, we demonstrate that human lymphocytes can take up and utilize murine lipoproteins: engrafted human PBL can participate in the mouse lipid metabolism, and an exchange of membrane lipids in vivo is, therefore, possible. Second, plasma membrane viscosity was determined before and after engraftment: before engraftment, PBL from the elderly showed a significantly higher membrane viscosity than that from young controls, but this difference vanished during engraftment into SCID mice, wherein cells from both age groups exhibited nearly identical values. It was, therefore, concluded that lymphocyte membrane viscosity is influenced by environmental factors, and that the age-related increase is, in principle, reversible. |
| Databáze: | OpenAIRE |
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