Development of analytical protocol for the investigation of transformation products of pre-harvest fungicides in fruits using LC-MS/MS methods
Autor: | Tamas Lamfalusy, Csilla Sörös |
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Rok vydání: | 2021 |
Předmět: |
Health
Toxicology and Mutagenesis Chemical protection Prunus avium 010501 environmental sciences Toxicology Mass spectrometry 01 natural sciences Tandem Mass Spectrometry Lc ms ms 0105 earth and related environmental sciences Chromatography Chemistry fungi 010401 analytical chemistry Public Health Environmental and Occupational Health food and beverages General Chemistry General Medicine Fungicides Industrial 0104 chemical sciences Fungicide Transformation (genetics) Fruit Malus Chemical agents Chromatography Liquid Food Science |
Zdroj: | Food Additives & Contaminants: Part A. 38:1206-1217 |
ISSN: | 1944-0057 1944-0049 |
Popis: | Chemical protection of plants is critical to permit all-year-long availability of plant products. These chemical agents are transformed both biotically and abiotically after spraying. Our purpose was to develop a workflow which is suitable to investigate those transformation products from plant matrices. Two field trials were set up in two years in two different plant matrices (apples, cherries) to develop a workflow to map the transformation products (TP) of three selected pre-harvest fungicides (boscalid, fluopyram, pyraclostrobin) in the fruits. Modified QuEChERS extraction method was applied for the extraction of TPs from the fruit matrices. We used liquid chromatograph-mass spectrometers to identify and confirm the transformation products of fungicides. LC-QTOF-MS method was suitable to map the key fragmentation routes of parent fungicides. Based on fragmentation pathways, MRM (multiple-reaction monitoring) transitions of fungicide-metabolites mentioned in the literature were predicted. HPLC-QTRAP-MS in target mode was successfully applied to monitor trace level of metabolites and in some cases their isomers. For confirmation of the identified metabolites, LC-QTOF-MS was used. Five earlier-documented as well as one novel transformation product (deschloro-FLP) were found in the investigated fruit samples, the latter has not been reported in plant matrices so far. Area-normalisation method was used to follow the relative concentration of the transformation products in the fruits as a function of time. |
Databáze: | OpenAIRE |
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