The Influence of Bifidobacterium bifidum and Bacteroides fragilis on Enteric Glial Cell–Derived Neurotrophic Factors and Inflammasome
| Autor: | Cai-Hua Yan, Peng-Chun Yang, Shi-Yu Li, Xiaohua Hou, Yan-Hua Yang, Wei Qian, Chibing Dai, Jing Wang, Xi-Jun Li, Qin Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine Lipopolysaccharide Inflammasomes Immunology ved/biology.organism_classification_rank.species Bacteroides fragilis Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Neurotrophic factors Glial cell line-derived neurotrophic factor medicine Animals Immunology and Allergy Glial Cell Line-Derived Neurotrophic Factor Inflammation NLRP6 Microscopy Confocal Bifidobacterium bifidum biology ved/biology Chemistry Probiotics Inflammasome biology.organism_classification Gastrointestinal Microbiome Rats Cell biology Intestines 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Neuroglia medicine.drug |
| Zdroj: | Inflammation. 43:2166-2177 |
| ISSN: | 1573-2576 0360-3997 |
| DOI: | 10.1007/s10753-020-01284-z |
| Popis: | Enteric glial cells (EGCs) and enteric glial-derived neurotrophic factor (GDNF) are directly involved in intestinal inflammation. In this study, we sought to examine the possible mechanisms for how Bifidobacterium bifidum (B.b.) and Bacteroides fragilis (B.f.) influence EGC regulation. In this study, lipopolysaccharide (LPS) and interferon-γ (IFN-γ) were used as exogenous stimuli of EGCs to establish an intestinal inflammation model. After stimulation with LPS and IFN-γ, B.b. and B.f. supernatants were used to activate EGCs and to examine EGC immune mechanisms. For this purpose, qRT-PCR, western blotting, and laser scanning confocal microscopy (LSCM) were used to detect the expression of NLRP3, NLRP6, NGF, NT-3, IL-18, IL-1β, and caspase-1. We found that EGCs, after stimulation with LPS and IFN-γ, could express NLRP3, NLRP6, NT-3, NGF, IL-18, IL-1β, and caspase-1 through LSCM. In intestinal inflammation, B.b. and B.f. could trigger an increase in NGF and NT-3 expression in EGCs in order to protect the intestine. Furthermore, B.b. and B.f. could upregulate NLRP3 expression in EGCs and promote an inflammatory response. B.b. had a dual regulatory role in EGC NLRP6 expression, while B.f. inhibited NLRP6 protein expression. Moreover, B.b. could decrease the expression of IL-18, IL-1β, and caspase-1 in EGCs in order to inhibit the inflammatory response. Contrary to this, B.f. could upregulate IL-18, IL-1β, and caspase-1 expression in EGCs in order to promote the inflammatory response. B.b. and B.f. can influence the expression of NGF, NT-3, NLRP3, NLRP6, IL-18, IL-1β, and caspase-1 in EGCs in order to inhibit or promote intestinal inflammation. |
| Databáze: | OpenAIRE |
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