Strong Hepatitis C Virus (HCV)–specific Cell-mediated Immune Responses in the Absence of Viremia or Antibodies Among Uninfected Siblings of HCV Chronically Infected Children
| Autor: | Hanaa El-Karaksy, Gamal Esmat, Heba Helmy, Zainab Z. Ali, Maha Sobhy, Suzan El-Naghi, Sayed F. Abdelwahab, Samer S. El-Kamary, G. Thomas Strickland, Gehan Galal, Mohamed D. Hashem, Mohamed T. Shata |
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| Rok vydání: | 2011 |
| Předmět: |
CD4-Positive T-Lymphocytes
Enzyme-Linked Immunospot Assay Adolescent Hepatitis C virus Hepacivirus chemical and pharmacologic phenomena Viremia medicine.disease_cause complex mixtures Virus Interferon-gamma Major Articles and Brief Reports Flaviviridae medicine Humans Immunology and Allergy Child Immunity Cellular biology Reverse Transcriptase Polymerase Chain Reaction Siblings ELISPOT virus diseases Hepatitis C Antibodies Hepatitis C Chronic bacterial infections and mycoses Flow Cytometry medicine.disease biology.organism_classification Virology digestive system diseases Infectious Diseases Child Preschool Immunology biology.protein RNA Viral Egypt Viral disease Hepatitis C Antigens Antibody |
| Zdroj: | The Journal of Infectious Diseases. 203:854-861 |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | Infection with hepatitis C virus (HCV) can result in an HCV-specific cell-mediated immune (CMI) response in the absence of detectable HCV antibodies (anti-HCV) and RNA [1]. CMI responses to HCV antigens have been found in HCV-seronegative individuals who may have been exposed to the virus, including heath care workers [2], spouses [3], household contacts of persons with HCV infection [1, 4], children born to HCV-infected women [5, 6], prisoners [7], and injection drug users [8–10]. Low infective doses of HCV given to chimpanzees have resulted in induction of HCV-specific interferon γ (IFN-γ) synthesis in the absence of persistent infection as determined by enzyme-linked immunospot (ELISPOT) assay [11]. Our group previously reported on CMI responses to HCV antigens in anti-HCV-negative aviremic individuals [4] residing in a rural Egyptian community, where the prevalence of anti-HCV was 24% [12, 13]. Among individuals living with at least 2 anti-HCV household family members, 18% had positive HCV-specific IFN-γ responses, compared with only 2.9% of those living with no seropositive household members (P = .03) [4]. These findings suggest that an effective CMI response may accelerate clearance of HCV infection in the absence of persisting antibodies and that CMI response could be used as a surrogate marker for prior transient HCV infection. In the present study, we analyzed the HCV-specific CMI responses in seronegative children, and some of their chronically infected siblings, who lived in the same households and were being followed up in a natural history study of HCV infection in Egyptian children. |
| Databáze: | OpenAIRE |
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