Postprandial Lipemia is Associated With Platelet and Monocyte Activation and Increased Monocyte Cytokine Expression in Normolipemic Men
| Autor: | Teresa Paglieroni, John C. Rutledge, Dianne A. Hyson, Theodore Wun |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Time Factors 030204 cardiovascular system & hematology Monocytes 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Cell Adhesion medicine Humans Ingestion Platelet Platelet activation Receptor Analysis of Variance Meal Triglyceride Tumor Necrosis Factor-alpha business.industry Monocyte Hematology General Medicine Middle Aged Platelet Activation Postprandial Period Dietary Fats Lipids Up-Regulation 030104 developmental biology Postprandial medicine.anatomical_structure Endocrinology chemistry Cytokines business Interleukin-1 |
| Zdroj: | Clinical and Applied Thrombosis/Hemostasis. 8:147-155 |
| ISSN: | 1938-2723 1076-0296 |
| DOI: | 10.1177/107602960200800211 |
| Popis: | The activation of platelets and monocytes has been implicated in the development of cardiovascular diseases. We asked the question if postprandial lipemia following a fat- containing meal is associated with platelet and monocyte activation and increased platelet-monocyte interaction. Thirteen healthy, normal weight, normolipemic males, 20 to 49 years, consumed a 40% fat meal of whole foods. Blood samples were obtained at fasting and 3 1/2 and 6 hours after ingestion. Triglyceride levels increased to 48% over baseline at 3 1/2 hours postconsumption and returned to fasting levels by 6 hours. Multiparameter flow cytometry using monoclonal antibodies showed that the percentage of platelets expressing surface P-selectin and the activated conformation the GPIIb-IIa receptor was significantly higher at 3 1/2 hours compared to fasting. The percentage of platelet-monocyte aggregates increased by 36% at 3 1/2 hours and 43% at 6 hours postconsumption. The percentage of monocytes expressing intracellular tumor necrosis factor-alpha (TNF-alpha) increased seven and eightfold at 3 1/2 and 6 hours, respectively. The expression of interleukin-1beta (IL-1beta increased in a similar manner. These data suggest activation of platelets and monocytes after a moderate fat meal. Repetitive activation of platelets and monocytes could be an early event in the initiation and development of atherosclerosis. |
| Databáze: | OpenAIRE |
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