Sequence analysis of bone morphogenetic protein receptor type II mRNA from ascitic and nonascitic commercial broilers
| Autor: | Nicholas B. Anthony, Annie M. Donoghue, C.R. Cisar, J. M. Balog |
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| Rok vydání: | 2003 |
| Předmět: |
Untranslated region
Sequence analysis Hypertension Pulmonary Gene Expression Single-nucleotide polymorphism Protein Serine-Threonine Kinases Biology Bone Morphogenetic Protein Receptors Type II Polymorphism Single Nucleotide Gene expression Animals Coding region Bone morphogenetic protein receptor RNA Messenger Codon Lung Gene Poultry Diseases Genetics Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis RNA Myocardium Broiler Ascites General Medicine Molecular biology Animal Science and Zoology Chickens |
| Zdroj: | Poultry Science. 82:1494-1499 |
| ISSN: | 0032-5791 |
| DOI: | 10.1093/ps/82.10.1494 |
| Popis: | Ascites syndrome, also known as pulmonary hypertension syndrome (PHS), is a common metabolic disorder in rapidly growing meat-type chickens. Environmental factors, such as cold, altitude, and diet, play significant roles in development of the disease, but there is also an important genetic component to PHS susceptibility. The human disease familial primary pulmonary hypertension (FPPH) is similar to PHS in broilers both genetically and physiologically. Several recent studies have shown that mutations in the bone morphogenetic protein receptor type II (BMPR2) gene are a cause of FPPH in humans. To determine whether mutations in the chicken BMPR2 gene play a similar role in PHS susceptibility, BMPR-II mRNA from ascitic and nonascitic commercial broilers were sequenced and compared with the published Leghorn chicken BMPR-II mRNA sequence. Fourteen single nucleotide polymorphisms (SNP) were identified in the commercial broiler BMPR-II mRNA. No mutations unique to ascites-susceptible broilers were present in the coding, 5' untranslated or 3' untranslated regions of BMPR-II mRNA. The twelve SNP present within the coding region of BMPR-II mRNA were synonymous substitutions and did not alter the BMPR-II protein sequence. In addition, analysis of BMPR2 gene expression by reverse transcriptase-PCR indicated that there were no differences in BMPR-II mRNA levels in ascitic and nonascitic birds. Therefore, it appears unlikely that mutations in the BMPR2 gene were responsible for susceptibility to PHS in these commercial broilers. |
| Databáze: | OpenAIRE |
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