Characterization of a Novel Fructosyltransferase from Lactobacillus reuteri That Synthesizes High-Molecular-Weight Inulin and Inulin Oligosaccharides
| Autor: | G.H. van Geel-Schutten, Hakim Rahaoui, S.A F T van Hijum, van der Marc Maarel, Lubbert Dijkhuizen |
|---|---|
| Přispěvatelé: | Groningen Biomolecular Sciences and Biotechnology, Host-Microbe Interactions |
| Rok vydání: | 2002 |
| Předmět: |
Sucrose
ENZYME PROTEINS Molecular Sequence Data Inulin Oligosaccharides LACTIC-ACID BACTERIA medicine.disease_cause SEQUENCE Applied Microbiology and Biotechnology chemistry.chemical_compound Fructan stomatognathic system Lactobacillus Escherichia coli medicine Amino Acid Sequence PURIFICATION Inulosucrase Sequence Homology Amino Acid Ecology biology Fructose biology.organism_classification STREPTOCOCCUS-MUTANS GENE Streptococcus mutans Recombinant Proteins Lactobacillus reuteri Molecular Weight body regions ALIGNMENT Hexosyltransferases chemistry Biochemistry Food Microbiology BACILLUS-SUBTILIS LEVANSUCRASE FRUCTAN Food Science Biotechnology |
| Zdroj: | Applied and environmental microbiology, 68(9), 4390-4398. AMER SOC MICROBIOLOGY |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.68.9.4390-4398.2002 |
| Popis: | Fructosyltransferase (FTF) enzymes produce fructose polymers (fructans) from sucrose. Here, we report the isolation and characterization of an FTF-encoding gene from Lactobacillus reuteri strain 121. A C-terminally truncated version of the ftf gene was successfully expressed in Escherichia coli . When incubated with sucrose, the purified recombinant FTF enzyme produced large amounts of fructo-oligosaccharides (FOS) with β-(2 → 1)-linked fructosyl units, plus a high-molecular-weight fructan polymer (>10 7 ) with β-(2 → 1) linkages (an inulin). FOS, but not inulin, was found in supernatants of L. reuteri strain 121 cultures grown on medium containing sucrose. Bacterial inulin production has been reported for only Streptococcus mutans strains. FOS production has been reported for a few bacterial strains. This paper reports the first-time isolation and molecular characterization of (i) a Lactobacillus ftf gene, (ii) an inulosucrase associated with a generally regarded as safe bacterium, (iii) an FTF enzyme synthesizing both a high molecular weight inulin and FOS, and (iv) an FTF protein containing a cell wall-anchoring LPXTG motif. The biological relevance and potential health benefits of an inulosucrase associated with an L. reuteri strain remain to be established. |
| Databáze: | OpenAIRE |
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