Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes
| Autor: | André Lipski, Vanessa Kombeitz, Alexander Flegler |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Membrane Fluidity
Acclimatization medicine.disease_cause Biochemistry Microbiology Bacterial cell structure Amino Acids Aromatic 03 medical and health sciences chemistry.chemical_compound Listeria monocytogenes Stress Physiological Genetics Aromatic amino acids Membrane fluidity medicine Food science Fatty acids Lipid bilayer Molecular Biology Pathogen 030304 developmental biology chemistry.chemical_classification Original Paper 0303 health sciences 030306 microbiology Chemistry Cold adaptation Fatty acid Vitamin K 2 General Medicine Bacterial cell fitness Cold Temperature Membrane Menaquinone |
| Zdroj: | Archives of Microbiology |
| ISSN: | 1432-072X 0302-8933 |
| DOI: | 10.1007/s00203-021-02322-6 |
| Popis: | Listeria monocytogenes is a food-borne pathogen with the ability to grow at low temperatures down to − 0.4 °C. Maintaining cytoplasmic membrane fluidity by changing the lipid membrane composition is important during growth at low temperatures. In Listeria monocytogenes, the dominant adaptation effect is the fluidization of the membrane by shortening of fatty acid chain length. In some strains, however, an additional response is the increase in menaquinone content during growth at low temperatures. The increase of this neutral lipid leads to fluidization of the membrane and thus represents a mechanism that is complementary to the fatty acid-mediated modification of membrane fluidity. This study demonstrated that the reduction of menaquinone content for Listeria monocytogenes strains resulted in significantly lower resistance to temperature stress and lower growth rates compared to unaffected control cultures after growth at 6 °C. Menaquinone content was reduced by supplementation with aromatic amino acids, which led to a feedback inhibition of the menaquinone synthesis. Menaquinone-reduced Listeria monocytogenes strains showed reduced bacterial cell fitness. This confirmed the adaptive function of menaquinones for growth at low temperatures of this pathogen. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink