Magnetic Field-Induced Alignment of Nanofibrous Supramolecular Membranes: A Molecular Design Approach to Create Tissue-like Biomaterials
| Autor: | Ian W. Hamley, Meagan S. Mauter, Xitong Liu, Michael J. Reece, Charlotte J. C. Edwards-Gayle, Valeria Castelletto, Yejiao Shi, Helena S. Azevedo, Salvatore Grasso, Elham Radvar |
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| Rok vydání: | 2020 |
| Předmět: |
Circular dichroism
Materials science Supramolecular chemistry Stacking Nanofibers 02 engineering and technology 010402 general chemistry 01 natural sciences Elastic Modulus Tensile Strength Cell Adhesion Humans General Materials Science Hyaluronic Acid Tissue Scaffolds Membranes Artificial Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Random coil 0104 chemical sciences Membrane Nanofiber Self-healing hydrogels Biophysics Self-assembly 0210 nano-technology Peptides |
| Zdroj: | ACS applied materialsinterfaces. 12(20) |
| ISSN: | 1944-8252 |
| Popis: | A molecular design approach to fabricate nanofibrous membranes by self-assembly of aromatic cationic peptides with hyaluronic acid (HA) and nanofiber alignment under a magnetic field is reported. Peptides are designed to contain a block composed of four phenylalanine residues at the C-terminus, to drive their self-assembly by hydrophobic association and aromatic stacking, and have a positively charged domain of lysine residues for electrostatic interaction with HA. These two blocks are connected by a linker with a variable number of amino acids and the ability to adopt distinct conformations. Zeta potential measurements and circular dichroism confirm their positive charge and variable conformation (random coil, β-sheet, or α-helix), which depend on the pH and sequence. Their self-assembly, examined by fluorescence spectroscopy, small-angle X-ray scattering, and transmission electron microscopy, show the formation of fiberlike nanostructures in the micromolar range. When the peptides are combined with HA, hydrogels or flat membranes are formed. The molecular structure tunes the mechanical behavior of the membranes and the nanofibers align in the direction of magnetic field due to the high diamagnetic anisotropy of phenylalanine residues. Mesenchymal stem cells cultured on magnetically aligned membranes elongate in direction of the nanofibers supporting their application for soft tissue engineering. |
| Databáze: | OpenAIRE |
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