HuR mRNA Ligands Expressed After Seizure
| Autor: | Virginia B. Bartleson, Saswati Bhattacharya, Françoise A. Gourronc, Cara J. Westmark, James S. Malter, Tom Sutula, Umit Sayin |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Cell signaling ELAV-Like Protein 1 Convulsants In Vitro Techniques Biology Ligands Hippocampus Pathology and Forensic Medicine Mice Cellular and Molecular Neuroscience Seizures Animals RNA Messenger Gene Transcription factor Gene Library AU-rich element Messenger RNA cDNA library RNA-Binding Proteins General Medicine Cell biology Mice Inbred C57BL ELAV Proteins Neurology Antigens Surface Pentylenetetrazole Neurology (clinical) Cell activation Neuroscience |
| Zdroj: | Journal of Neuropathology and Experimental Neurology. 64:1037-1045 |
| ISSN: | 0022-3069 |
| DOI: | 10.1097/01.jnen.0000189835.71574.e1 |
| Popis: | HuR is a ubiquitously expressed AU-rich element (ARE)-binding protein that interacts with and stabilizes selective early response gene (ERG) mRNAs after cell activation or stress. To date, approximately 20 mRNAs have been unambiguously defined as HuR ligands. Given the discordance between the large number of ERG mRNAs and those few defined as ligands, we applied in vitro selection to isolate a broad range of HuR mRNA ligands using postseizure mouse hippocampal tissue. Selected mRNAs were converted into cDNA libraries and sequenced. Using this approach, we have identified over 600 novel, putative HuR mRNA ligands. These genes code for a variety of proteins, including transcription factors, signaling molecules, and kinases, but many have unknown function. Consistent with the means of their selection, several of these HuR ligands are differentially expressed in hippocampus after seizure. These results demonstrate a biochemical approach to identify and characterize the diverse repertoire of ligands for HuR and other regulatory mRNA-binding proteins. |
| Databáze: | OpenAIRE |
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