Effects of rikkunshito supplementation on resistance to oxidative stress and lifespan in mice
| Autor: | Yukari Watanabe, Motoyasu Satou, Zi Wang, Yoshihisa Ohata, Seongjoon Park, Takuya Chiba, Toshimitsu Komatsu, Yoshitaka Kondo, Yiwen Yuan, Isao Shimokawa, Yuki Yoshii, Ryoichi Mori |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Normal diet Longevity Calorie restriction medicine.disease_cause Mice Downregulation and upregulation Internal medicine medicine Animals Doxorubicin Caloric Restriction Mice Knockout business.industry Neuropeptide Y receptor Ghrelin Oxidative Stress Endocrinology Dietary Supplements Knockout mouse Female business Oxidative stress Drugs Chinese Herbal medicine.drug |
| Zdroj: | Geriatrics & Gerontology International. 20:238-247 |
| ISSN: | 1447-0594 1444-1586 |
| DOI: | 10.1111/ggi.13848 |
| Popis: | Aim Caloric restriction (CR), which limits the caloric intake to 60-70% of ad libitum (AL) amounts in various experimental animals, delays aging and extends the lifespan. We previously showed that neuropeptide Y (NPY), an appetite-stimulating peptide, is essential for the anti-oxidative and life-extending effects of CR. Here, we investigated whether a Japanese traditional herbal medicine, rikkunshito (RKT), which induces NPY activation, has CR-like life-extending effects. Methods First, we evaluated the life-extending activity of RKT by examining the effect of long-term RKT administration on wild-type and NPY knockout mice. Furthermore, we tested whether RKT enhances CR-mediated beneficial effects under AL conditions with a normal diet and under mild CR conditions with a high-fat diet. We then used 3-nitropropionic acid or doxorubicin to induce oxidative stress, and analyzed the differences in survival rate, weight loss, gene expression and cellular oxidative damage among groups. Results RKT administration did not extend the lifespan of wild-type or NPY knockout mice. In the oxidative stress models, RKT treatment upregulated anti-oxidative gene expression in the liver. Furthermore, RKT administration reduced the oxidative damage in the liver compared to the CR conditions alone. However, on induction of oxidative stress by 3-nitropropionic acid or doxorubicin, RKT administration did not affect the survival rate. Conclusions These results show that RKT administration only partially mimics the effects of CR at the cellular level, but not at the organismal level to increase the lifespan of mice. Geriatr Gerontol Int 2019; ••: ••-••. |
| Databáze: | OpenAIRE |
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