Chemically induced hypoxia by dimethyloxalylglycine (DMOG)-loaded nanoporous silica nanoparticles supports endothelial tube formation by sustained VEGF release from adipose tissue-derived stem cells
| Autor: | Axel Haverich, Florian Helms, Peter Behrens, Melanie Klingenberg, Mathias Wilhelmi, Nina Ehlert, Anne Lyons, Karen F W Besecke, Sarah Zippusch, Ulrike Böer |
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| Rok vydání: | 2021 |
| Předmět: |
Tube formation
Endothelium nanoporous silica nanoparticles Adipose tissue pre-vascularization dimethyloxalylglycine Umbilical vein Cell biology Biomaterials Vascular endothelial growth factor chemistry.chemical_compound Vascular endothelial growth factor A medicine.anatomical_structure chemistry Tissue engineering tissue engineering adipose tissue-derived stem cells medicine AcademicSubjects/SCI01410 Stem cell AcademicSubjects/MED00010 Research Article |
| Zdroj: | Regenerative Biomaterials |
| ISSN: | 2056-3426 2056-3418 |
| Popis: | Inadequate vascularization leading to insufficient oxygen and nutrient supply in deeper layers of bioartificial tissues remains a limitation in current tissue engineering approaches to which pre-vascularization offers a promising solution. Hypoxia triggering pre-vascularization by enhanced vascular endothelial growth factor (VEGF) expression can be induced chemically by dimethyloxalylglycine (DMOG). Nanoporous silica nanoparticles (NPSNPs, or mesoporous silica nanoparticles, MSNs) enable sustained delivery of molecules and potentially release DMOG allowing a durable capillarization of a construct. Here we evaluated the effects of soluble DMOG and DMOG-loaded NPSNPs on VEGF secretion of adipose tissue-derived stem cells (ASC) and on tube formation by human umbilical vein endothelial cells (HUVEC)-ASC co-cultures. Repeated doses of 100 µM and 500 µM soluble DMOG on ASC resulted in 3- to 7-fold increased VEGF levels on day 9 (P |
| Databáze: | OpenAIRE |
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