Comparison between Clinical Aspects and Salivary Microbial Profile of Children with and without Early Childhood Caries: A Preliminary Study
| Autor: | Andréa Gonçalves Antonio, Leandro Araujo Lobo, K.C. Pinto, ES Pires, Lucianne Cople Maia, Aline Borburema Neves, Mep Requejo |
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| Rok vydání: | 2015 |
| Předmět: |
Male
media_common.quotation_subject Microbial Consortia Breastfeeding Colony Count Microbial Clinical exam Dentistry Biology Dental Caries Polymerase Chain Reaction Streptococcus mutans Hygiene Lactobacillus medicine Humans Saliva Physical Examination media_common Candida Anamnesis business.industry DMF Index Denaturing Gradient Gel Electrophoresis General Medicine medicine.disease biology.organism_classification Oral Hygiene Bacterial Load Bottle Feeding Diet Pacifiers stomatognathic diseases Breast Feeding Child Preschool Pacifier Female business Early childhood caries |
| Zdroj: | The Journal of clinical pediatric dentistry. 39(3) |
| ISSN: | 1053-4628 |
| Popis: | Purpose: To compare the clinical aspects and microbial profile of children with and without early childhood caries (ECC). Study design: 14 patients (7 without caries and 7 with ECC) were submitted to anamnesis, clinical exam and saliva collection for microbiological analyses. Counts of Streptococcus mutans, Lactobacillus spp. Candida spp., and total microorganisms were performed by culture methods. Microbial diversity was characterized by PCR-DGGE. Demographic/clinical data and salivary microbial counts were compared between groups. Results: Habits of hygiene and breastfeeding presented no association with ECC. Use of pacifiers was associated with absence of caries (p=0.035). Counts of total microorganisms and Candida spp. did not differ between the groups. The ECC group presented larger quantity of S. mutans (p=0.026) and Lactobacillus spp. (p=0.002). There was no correlation between microorganisms and breastfeeding and pacifier use. The dmf-t of ECC Group was 10.5±1.9 and the modified dmf-t was 11.3±3.6. The DGGE demonstrated difference in the pattern of bands between the groups. Conclusion: Pacifiers usage was associated with the absence of ECC and microorganism number was higher in the caries group. The PCR-DGGE revealed a characteristic microbial diversity in the ECC Group, being an excellent tool for observing the dynamics of the salivary microbial community in these patients. |
| Databáze: | OpenAIRE |
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