Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment
| Autor: | Jay-Shake Li, Yung-I Lou, Che-Yu Lin, Yung-Chih Kuo |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pharmaceutical Science 02 engineering and technology blood–brain barrier Hippocampus Receptor tyrosine kinase chemistry.chemical_compound Drug Delivery Systems International Journal of Nanomedicine Drug Discovery Cardiolipin Phosphorylation Original Research Neurons β-amyloid Kinase Neurodegeneration neurodegeneration Brain General Medicine 021001 nanoscience & nanotechnology Immunohistochemistry medicine.anatomical_structure Biochemistry liposome 0210 nano-technology Alzheimer’s disease Curcumin Cardiolipins Cell Survival Wheat Germ Agglutinins p38 mitogen-activated protein kinases Static Electricity Biophysics tau Proteins Bioengineering Biology Fluorescence Biomaterials 03 medical and health sciences Alzheimer Disease medicine Animals Humans Particle Size Rats Wistar Amyloid beta-Peptides Nervous tissue wheat germ agglutinin Organic Chemistry JNK Mitogen-Activated Protein Kinases medicine.disease Molecular biology Wheat germ agglutinin Kinetics stomatognathic diseases 030104 developmental biology Nerve growth factor chemistry Liposomes Nerve Degeneration biology.protein |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| DOI: | 10.2147/ijn.s128396 |
| Popis: | Yung-Chih Kuo,1 Che-Yu Lin,1 Jay-Shake Li,2 Yung-I Lou3 1Department of Chemical Engineering, 2Department of Psychology, National Chung Cheng University, Chia-Yi, 3Department of Accounting, Providence University, Taichung, Taiwan, Republic of China Abstract: Curcumin (CRM) and nerve growth factor (NGF) were entrapped in liposomes (LIP) with surface wheat germ agglutinin (WGA) to downregulate the phosphorylation of kinases in Alzheimer’s disease (AD) therapy. Cardiolipin (CL)-conjugated LIP carrying CRM (CRM-CL/LIP) and also carrying NGF (NGF-CL/LIP) were used with AD models of SK-N-MC cells and Wistar rats after an insult with β-amyloid peptide (Aβ). We found that CRM-CL/LIP inhibited the expression of phosphorylated p38 (p-p38), phosphorylated c-Jun N-terminal kinase (p-JNK), and p-tau protein at serine202 and prevented neurodegeneration of SK-N-MC cells. In addition, NGF-CL/LIP could enhance the quantities of p-neurotrophic tyrosine kinase receptor type 1 and p-extracellular signal-regulated kinase 5 for neuronal rescue. Moreover, WGA-grafted CRM-CL/LIP and WGA-grafted NGF-CL/LIP significantly improved the permeation of CRM and NGF across the blood–brain barrier, reduced Aβ plaque deposition and the malondialdehyde level, and increased the percentage of normal neurons and cholinergic activity in the hippocampus of AD rats. Based on the marker expressions and in vivo evidence, current LIP carriers can be promising drug delivery systems to protect nervous tissue against Aβ-induced apoptosis in the brain during the clinical management of AD. Keywords: liposome, Alzheimer’s disease, β-amyloid, neurodegeneration, blood–brain barrier, wheat germ agglutinin |
| Databáze: | OpenAIRE |
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