New Potential Biocatalysts by Laccase Immobilization in PVA Cryogel Type Carrier
| Autor: | Magdalena Fogorasi, Boris Shaskolskiy, Simona Gavrilas, Michaela Dina Stanescu, Vladimir I. Lozinsky |
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| Rok vydání: | 2009 |
| Předmět: |
Vinyl alcohol
Immobilized enzyme Surface Properties Bioengineering Applied Microbiology and Biotechnology Biochemistry Polyvinyl alcohol chemistry.chemical_compound Organic chemistry Particle Size Molecular Biology Laccase Industrial scale Hydrogels General Medicine Hydrogen-Ion Concentration Enzymes Immobilized equipment and supplies Combinatorial chemistry Enzyme Activation chemistry Biocatalysis Covalent bond Polyvinyl Alcohol Self-healing hydrogels Porosity Cryogels Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 160:1947-1954 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-009-8755-0 |
| Popis: | Laccases are enzymes belonging to the Oxidoreductases class. These enzymes may be good biocatalysts for different processes, at laboratory and industrial levels. A successful use at industrial scale demands a higher stability of the enzyme. As an easy way to obtain longer life biocatalysts, the immobilization process is recommended. Thus, the paper presents different ways of obtaining new biocatalysts by a laccase covalent immobilization on a macroporous carrier based on poly(vinyl alcohol) cryogel. Different procedures of covalent immobilization are described, the newly obtained biocatalysts being characterized. According to the experimental data, the stability of the immobilized enzyme increased and the pH profile changed, compared with those of the free enzyme. |
| Databáze: | OpenAIRE |
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