Comparison of in vitro and in vivo protein release from hydrogel systems
| Autor: | Muhammad Badar, Stefanie Wöhl-Bruhn, Henning Menzel, Brigitte Tiersch, Heike Bunjes, Peter P. Mueller, Andreas Bertz, Joachim Koetz |
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| Přispěvatelé: | Technische Universität Braunschweig, Institute of Pharmaceutical Technology, Mendelssohnstraße 1, 38106 Braunschweig, Germany. |
| Rok vydání: | 2012 |
| Předmět: |
Male
Kinetics Pharmaceutical Science Nanotechnology Hydroxyethyl starch Methacrylate Hydrogel Polyethylene Glycol Dimethacrylate Polyethylene Glycols Hydroxyethyl Starch Derivatives Mice Drug Delivery Systems In vivo Absorbable Implants medicine Animals Humans reproductive and urinary physiology Hetastarch chemistry.chemical_classification Mice Inbred BALB C Goats Polymer In vitro Hydrogel chemistry Immunoglobulin G Biophysics Institut für Chemie Methacrylates biological phenomena cell phenomena and immunity Fluorescein-5-isothiocyanate Preclinical imaging medicine.drug |
| Zdroj: | Journal of Controlled Release. 162:127-133 |
| ISSN: | 0168-3659 |
| DOI: | 10.1016/j.jconrel.2012.05.049 |
| Popis: | Hydrogel systems based on hydroxyethyl starch-polyethylene glycol methacrylate (HES-P(EG)(6)MA) or hydroxyethyl starch methacrylate (HES-MA) were used to assess the protein release behavior. Here, we analyzed the in vitro release of FITC-anti-human antibodies incorporated in either HES-P(EG)(6)MA or HES-MA hydrogel delivery systems in PBS or human serum. In addition, hydrogel disks and microparticles prepared from the two polymers were subcutaneously implanted in BALB/c mice. The in vivo release of FITC-IgG was non-invasively monitored by an in vivo imaging system (IVIS 200) over a time period of up to 3 months. The imaging system allowed to asses individual animals over time, therefore only a small number of animals was required to obtain high quality data. The reduction in fluorescence intensity at the site of administration was compared to in vitro release profiles. These investigations demonstrated a sustained release from HES-MA hydrogel disks compared to rapidly degrading HES-P(EG)(6)MA disks and microparticles. The sustained release from HES-MA disks could be further optimized by using increased polymer concentrations. Human serum as in vitro release medium reflected better the in vivo release from HES-P(EG)(6)MA systems than PBS, suggesting that the presence of organic substances like proteins or lipids may play a significant role for the release kinetics. |
| Databáze: | OpenAIRE |
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