Mercury accumulation and its effects on molecular, physiological, and histopathological responses in the peacock blenny Salaria pavo

Autor: Patrick Kestemont, Zohra Haouas, Azza Naïja, Ahmed Noureddine Helal, Ronny Blust, Justine Marchand, Benoît Chénais
Přispěvatelé: Bioressources: Integrative Biology and Valuation, Higher Institute of Biotechnology of Monastir (BIOLIVAL), Mer, molécules et santé EA 2160 (MMS), Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes (UN)-Université de Nantes (UN)-Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN), Research Unit in Environmental and Evolutionary Biology (URBE-NARILIS), Université de Namur [Namur] (UNamur), Research Unit of Genetic, Laboratory of Histology and Cytogenetic, Faculté de Médecine Dentaire de Monastir [Tunisie] (FMDM), Systemic Physiological and Ecotoxicological Research (SPHERE), Université d'Anvers (Universiteit Antwerpen), Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Gill
Gills
Salaria
Antioxidant
Health
Toxicology and Mutagenesis
medicine.medical_treatment
010501 environmental sciences
01 natural sciences
Toxicology
Lipid peroxidation
chemistry.chemical_compound
oxidative stress
Tissue Distribution
Peacock blenny
Hg accumulation
biology
q-RT-PCR
ABCB1
General Medicine
Pollution
Chemistry
Liver
Mercuric Chloride
ABC transporter
[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
medicine.medical_specialty
Tunisia
mercury
animal structures
Salaria pavo
HgCl
Histopathology
sentinel species
Biomarker activities
03 medical and health sciences
Internal medicine
medicine
Environmental Chemistry
Ecotoxicology
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
14. Life underwater
Biology
0105 earth and related environmental sciences
mRNA expression
Aquatic animal
Biomarker
biology.organism_classification
Perciformes
030104 developmental biology
Endocrinology
chemistry
13. Climate action
HgCl2
bioindicators
Water Pollutants
Chemical
Zdroj: Environmental Science and Pollution Research
Environmental Science and Pollution Research, Springer Verlag, 2016, 23 (21), pp.22099-22115. ⟨10.1007/s11356-016-7401-y⟩
ISSN: 1614-7499
0944-1344
DOI: 10.1007/s11356-016-7401-y⟩
Popis: For humans, fish consumption is the major source of mercury (Hg) exposure. The aim of this study was to assess the effect of Hg in the peacock blenny Salaria pavo, a species of the family of blennies that was used as indicator of water pollution. We performed a sublethal contamination of fish to 66 μg HgCl2 L−1 during 1, 4, 10 and 15 days but Hg concentration measured in the experimental water was much lower than the nominal concentration. Hg was also measured in both gill and liver tissues and displays a significant increase of its concentration in gills after 1 day of exposure followed by a decrease throughout the experiment. In the liver, Hg burden reaches its maximum at day 4 followed also by a decrease. Partial-length cDNA of mt1, mt2, gpx, cat, mnsod and cuznsod was characterized. Results from mRNA expression levels displayed an up-regulation of mt1, gpx and mnsod while a downregulation of cat was observed. Several biomarker activities were determined in gills and liver and exposure to Hg affected all antioxidant enzymes in gills. EROD, GST and GPx significantly decreased, while CAT levels increased from 4 days of Hg exposure. No lipid peroxidation (LPO) induction was observed in gills of exposed fish. Regarding the liver, the activity of all enzymes increased significantly from the beginning of the experiment. LPO induction was, however, induced after 4 days only. The histological analysis also performed indicated that fish exhibited several damages in gills and liver, mainly in relation to circulatory disturbances in the gills and regressive changes in the liver. All biomarkers assessed showed that peacock blennies are able to detoxify Hg from gill and liver tissues by developing various defense mechanisms.
Databáze: OpenAIRE
Externí odkaz: