Gut microbial features can predict host phenotype response to protein deficiency
| Autor: | Lara R. Dugas, Brian T. Layden, Anukriti Sharma, Guadalupe Navarro, Terrence Forrester, Jack A. Gilbert |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Nitrogen balance Low protein Methyltransferase Physiology Medical Physiology Gut flora nitrogen balance Inbred C57BL Oral and gastrointestinal Mice 0302 clinical medicine Lactobacillus 2.1 Biological and endogenous factors Bifidobacterium Original Research 2. Zero hunger 0303 health sciences biology Protein catabolism Phenotype 030211 gastroenterology & hepatology Roseburia medicine.symptom medicine.medical_specialty Nitrogen Clinical Sciences Immunology Gut microbiota malnutrition protein deficiency Excretion 03 medical and health sciences Physiology (medical) Internal medicine medicine Animals Microbiome Obesity 030304 developmental biology Nutrition 030109 nutrition & dietetics Ruminococcus Metabolism biology.organism_classification Gastrointestinal Microbiome Mice Inbred C57BL 030104 developmental biology Endocrinology Gastrointestinal Hepatic and Pancreatic Physiology Weight gain |
| Zdroj: | Physiological Reports Physiological reports, vol 6, iss 23 |
| ISSN: | 2051-817X |
| Popis: | Malnutrition remains a major health problem in low and middle income countries. During low protein intake, < 0.67 g/kg/day, there is a loss of nitrogen (N2) balance, due to the unavailability of amino acid for metabolism and unbalanced protein catabolism results. However, there are individuals, who consume the same low protein intake, and preserve N2 balance for unknown reasons. A novel factor, the gut microbiota, may account for these N2 balance differences. To investigate this, we correlated gut microbial profiles with the growth of four murine strains (C57Bl6/J, CD-1, FVB, and NIH-Swiss) on protein deficient (PD) diet. Results show that a PD diet exerts a strain-dependent impact on growth and N2 balance as determined through analysis of urinary urea, ammonia and creatinine excretion. Bacterial alpha diversity was significantly (p < 0.05, FDR) lower across all strains on a PD diet compared to normal chow (NC). Multi-group analyses of the composition of microbiomes (ANCOM) revealed significantly differential microbial signatures between the four strains independent of diet. However, mice on a PD diet demonstrated differential enrichment of bacterial genera including, Allobaculum (C57Bl6/J), Parabacteroides (CD-1), Turicibacter (FVB), and Mucispirillum (NIH-Swiss) relative to NC. Additionally, statistical model fitting revealed that the relative abundance of genera such as Bifidobacterium, Ruminococcus, and Lactobacillus were significantly positively correlated with body weight, while Anaerofustis, Roseburia, and Bilophila were significantly positively correlated with ammonia excretion. Taken together, these results suggest a potential relationship between the specific gut microbiota, N2 balance and animal response to malnutrition. |
| Databáze: | OpenAIRE |
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