Recombinant Mycobacterium tuberculosis KatG(S315T) is a competent catalase-peroxidase with reduced activity toward isoniazid

Autor:	Frank Rusnak, Nancy L. Wengenack, Frank R. Cockerill, James R. Uhl, Allison L. St. Amand, Bruce C. Kline, Stephen Naylor, Linda M. Benson, Andy J. Tomlinson
Rok vydání:	1997
Předmět:	Molecular Sequence Data medicine.disease_cause Cofactor Microbiology Mycobacterium tuberculosis chemistry.chemical_compound Bacterial Proteins medicine Escherichia coli Isoniazid Immunology and Allergy Amino Acid Sequence Heme Catalase-peroxidase Chromatography High Pressure Liquid chemistry.chemical_classification biology biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Catalase Recombinant Proteins Infectious Diseases Enzyme chemistry Peroxidases biology.protein Mutagenesis Site-Directed bacteria medicine.drug Peroxidase
Zdroj:	The Journal of infectious diseases. 176(3)
ISSN:	0022-1899
Popis:	The presence of KatG(S315T), a mutation frequently detected in clinical isolates of Mycobacterium tuberculosis, has been associated with loss of catalase-peroxidase activity and resistance to isoniazid therapy. Wild-type KatG and KatG(S315T) were expressed in a heterologous host (Escherichia coli) and purified to homogeneity, and enzymatic activity was measured. The catalase activity for KatG(S315T) was reduced 6-fold, and its peroxidase activity was decreased
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a18603cf1fdae334f5f2de13f92e488 https://pubmed.ncbi.nlm.nih.gov/9291321 Zobrazit plný text záznamu