Phylogenetic MLSA and phenotypic analysis identification of three probable novel Pseudomonas species isolated on King George Island, South Shetland, Antarctica
| Autor: | Felipe Vásquez-Ponce, Jorge Olivares-Pacheco, María S. Pavlov, Sergio H. Marshall, Sebastián Higuera-Llantén |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Pseudomonas antarctica P. fluorescens complex Genotype Sequence analysis Pseudomonas mandelii 030106 microbiology lcsh:QR1-502 Antarctic Regions Pseudomonas fluorescens DNA Ribosomal Microbiology lcsh:Microbiology 03 medical and health sciences Genus Multilocus Sequence Analysis RNA Ribosomal 16S Pseudomonas Phylogeny Soil Microbiology Islands Genetics Phylogenetic tree biology 16S ribosomal RNA biology.organism_classification Phenotype 030104 developmental biology Antarctica Research Paper Multilocus Sequence Typing |
| Zdroj: | Brazilian Journal of Microbiology v.49 n.4 2018 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Vol 49, Iss 4, Pp 695-702 Brazilian Journal of Microbiology, Volume: 49, Issue: 4, Pages: 695-702, Published: DEC 2018 |
| Popis: | Antarctica harbors a great diversity of microorganisms, including bacteria, archaea, microalgae and yeasts. The Pseudomonas genus is one of the most diverse and successful bacterial groups described to date, but only eight species isolated from Antarctica have been characterized. Here, we present three potentially novel species isolated on King George Island. The most abundant isolates from four different environments, were genotypically and phenotypically characterized. Multilocus sequence analysis and 16S rRNA gene analysis of a sequence concatenate for six genes (16S, aroE, glnS, gyrB, ileS and rpoD), determined one of the isolates to be a new Pseudomonas mandelii strain, while the other three are good candidates for new Pseudomonas species. Additionally, genotype analyses showed the three candidates to be part of a new subgroup within the Pseudomonas fluorescens complex, together with the Antarctic species Pseudomonas antarctica and Pseudomonas extremaustralis. We propose terming this new subgroup P. antarctica. Likewise, phenotypic analyses using API 20 NE and BIOLOG® corroborated the genotyping results, confirming that all presented isolates form part of the P. fluorescens complex. Pseudomonas genus research on the Antarctic continent is in its infancy. To understand these microorganisms’ role in this extreme environment, the characterization and description of new species is vital. |
| Databáze: | OpenAIRE |
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