Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1
| Autor: | Mei Lin, Glenn Steele, Cristina Mottola-Hartshorn, Thomas W. Smith, A. P. Chen, Martin Reers, Stephen T. Smiley, Lan Bo Chen |
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| Rok vydání: | 1991 |
| Předmět: |
Chemical Phenomena
Iodide Fluorescence spectrometry In Vitro Techniques Mitochondrion Biology Cell Line Membrane Potentials Mice Delocalized electron Cations Cricetinae Animals Inner mitochondrial membrane Fluorescent Dyes Membrane potential chemistry.chemical_classification Multidisciplinary Chemistry Physical Intracellular Membranes Carbocyanines Lipids Mitochondria Spectrometry Fluorescence Membrane Microscopy Fluorescence Solubility Biochemistry chemistry Biophysics Benzimidazoles Intracellular Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 88:3671-3675 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.88.9.3671 |
| Popis: | By using a potential-dependent J-aggregate-forming delocalized lipophilic cation, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine++ + iodide (JC-1), we find that membrane potentials across mitochondria in a living cell can be heterogeneous. Remarkably, even within a long contiguous mitochondrion, regional heterogeneity in membrane potentials appears to be possible. |
| Databáze: | OpenAIRE |
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