Increase in CD230 (cellular prion protein) fluorescence on blood lymphocytes in bovine spongiform encephalopathy-infected nonhuman primates
| Autor: | Gerhard Hunsmann, Uwe Hahmann, Barbara Yutzy, Andreas W. Stuke, Johannes Löwer, Andreas Hoffmann, Mechthild Törner, Edgar Holznagel, Kay-Martin Hanschman, Cheick Coulibaly, Walter J. Schulz-Schaeffer |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Time Factors Blood transfusion Lymphoid Tissue animal diseases Lymphocyte medicine.medical_treatment Bovine spongiform encephalopathy Immunology Biology Immunophenotyping Injections Flow cytometry Mice mental disorders medicine Animals Humans Immunology and Allergy PrPC Proteins Lymphocytes Whole blood Mice Knockout Infectivity medicine.diagnostic_test Age Factors Antibodies Monoclonal Brain Reproducibility of Results Hematology Middle Aged Flow Cytometry medicine.disease Virology Specific Pathogen-Free Organisms nervous system diseases Encephalopathy Bovine Spongiform Macaca fascicularis medicine.anatomical_structure Lymphatic system Blood Preservation Cattle Female |
| Zdroj: | Transfusion. 50:452-466 |
| ISSN: | 1537-2995 0041-1132 |
| Popis: | BACKGROUND: The cellular prion protein (PrPc) plays a central role in prion diseases such as variant Creutzfeldt-Jakob disease. This disease can be transmitted by blood transfusion. However, the exact kinetics of blood infectivity and the blood fraction carrying infectivity have not yet been identified. STUDY DESIGN AND METHODS: Simian PrPc epitopes were mapped by flow cytometry using monoclonal antibodies (MoAbs). A whole blood/no wash protocol was established, validated, and applied to investigate peripheral blood cell–associated PrPc expression profiles in bovine spongiform encephalopathy (BSE)-infected cynomolgus monkeys and age-/sex-matched controls. In addition, physiologic expression patterns on blood cells and in lymphoid tissues were determined. RESULTS: In BSE-infected macaques, blood lymphocyte–associated PrPc fluorescence gradually increased years before the onset of clinical signs (pF test |
| Databáze: | OpenAIRE |
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