Pinto beans modulate the gut microbiome, augment MHC II protein, and antimicrobial peptide gene expression in mice fed a normal or western-style diet

Autor: Bryant H. Keirns, Winyoo Chowanadisai, Matthew D. Hart, Babajide Ojo, Edralin A. Lucas, Guadalupe Davila El-Rassi, Jerry W. Ritchey, Grace Gallucci, Stephen L. Clarke, Peiran Lu, Dingbo Lin, Kendall L Anderson, Brenda J. Smith, Sanmi Alake
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Pore Forming Cytotoxic Proteins
medicine.medical_specialty
Endocrinology
Diabetes and Metabolism
Clinical Biochemistry
Antimicrobial peptides
Genes
MHC Class II
Adipose tissue
Gene Expression
Intra-Abdominal Fat
Major histocompatibility complex
Biochemistry
Impaired glucose tolerance
Butyric acid
03 medical and health sciences
chemistry.chemical_compound
Feces
Mice
0302 clinical medicine
Internal medicine
Gene expression
medicine
Animals
Humans
Obesity
Molecular Biology
Cecum
Phaseolus
Nutrition and Dietetics
biology
Lachnospiraceae
medicine.disease
Fatty Acids
Volatile
Gastrointestinal Microbiome
Nitric oxide synthase
Mice
Inbred C57BL
030104 developmental biology
Endocrinology
chemistry
Diet
Western
Dietary Supplements
biology.protein
Dysbiosis
030217 neurology & neurosurgery
Zdroj: The Journal of nutritional biochemistry. 88
ISSN: 1873-4847
Popis: The onset of type 2 diabetes in obesity is associated with gut dysbiosis and a failure to confine commensal bacteria and toxins to the gut lumen while prebiotics may prevent these effects. This study evaluated the effects of pinto beans (PB) supplementation on cecal bacteria, short-chain fatty acids (SCFAs), distal ileal antigen presentation marker (major histocompatibility complex [MHC] II) and antimicrobial peptide genes during short-term high-fat, high sucrose (HFS) feeding. Six-week-old, male C57BL/6J mice were randomly assigned to four groups (n=12/group), and fed a control (C) or HFS diet with or without cooked PB (10%, wt/wt) for 30 days. Supplemental PB in both the C and HFS diets decreased the abundance of Tenericutes and the sulfate-reducing bacteria Bilophila. In contrast, PB raised the abundance of taxa within the SCFAs-producing family, Lachnospiraceae, compared to groups without PB. Consequently, fecal butyric acid was significantly higher in PB-supplemented groups compared to C and HFS groups. PB reversed the HFS-induced ablation of the distal ileal STAT3 phosphorylation, and up-regulated antimicrobial peptide genes (Reg3γ and Reg3β). Furthermore, the expression of MHC II protein was elevated in the PB supplemented groups compared to C and HFS. Tenericutes and Bilophilia negatively correlated with activated STAT3 and MHC II proteins. Finally, supplemental PB improved fasting blood glucose, glucose tolerance and suppressed TNFα and inducible nitric oxide synthase mRNA in the visceral adipose tissue. Put together, the beneficial impact of PB supplementation on the gut may be central to its potential to protect against diet-induced inflammation and impaired glucose tolerance.
Databáze: OpenAIRE
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