The predictive value and potential mechanisms of miRNA-328 and miRNA-378 for brain metastases in operable and advanced non-small-cell lung cancer
| Autor: | Lan Ye, Yuhua Jiang, Huaying Hou, Jie Liu, Ying Xu, D.L. Sun, Maoqiang Ma, Xin Li, Xiaomei Li, Cuihong Wang |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Cancer Research Pathology medicine.medical_specialty Lung Neoplasms Protein Kinase C-alpha S100 Calcium Binding Protein beta Subunit Real-Time Polymerase Chain Reaction Gene Expression Regulation Enzymologic Tissue Culture Techniques chemistry.chemical_compound Predictive Value of Tests Carcinoma Non-Small-Cell Lung microRNA Biomarkers Tumor Medicine Humans Radiology Nuclear Medicine and imaging Protein kinase A Lung cancer Aged business.industry Brain Neoplasms General Medicine Middle Aged medicine.disease Cadherins Immunohistochemistry Vascular endothelial growth factor Gene Expression Regulation Neoplastic Vascular endothelial growth factor A MicroRNAs Real-time polymerase chain reaction Oncology chemistry Predictive value of tests Female business |
| Zdroj: | Japanese journal of clinical oncology. 45(5) |
| ISSN: | 1465-3621 |
| Popis: | Objective The incidence of brain metastases greatly varies in patients with non-small-cell lung cancer, and molecular markers are considered to predict brain metastases. Therefore, this study sought to identify the predictive value and potential mechanisms of miRNA-328 and miRNA-378 for brain metastases in non-small-cell lung cancer. Methods Patients who received a curable surgery for their lung cancer were screened according to our criteria. Formalin-fixed paraffin-embedded samples from the patients were examined for the expression of miRNA-328 and miRNA-378 using real-time polymerase chain reaction and the expression of N-cadherin, E-cadherin, vascular endothelial growth factor, protein kinase Cα and S100B were investigated using immunohistochemical staining. Results In total, 86 patients were screened for this study and 23 patients were diagnosed with brain metastases during the follow-up period. Comparing patients with and without brain metastases, the expression of miRNA-328 and miRNA-378 in tumor tissues were significantly different (P = 6.2 × 10(-5) and P = 2.8 × 10(-5), respectively). For the patients with brain metastases, the expression of miRNA-328 and miRNA-378 in tumor tissues compared with para-carcinoma tissues were also significantly different (P = 2.2 × 10(-5) and P = 1.6 × 10(-5), respectively). For patients with brain metastases, the association between miRNA-328 and protein kinase Cα was significant (r = 0.591, P = 0.003), but that between miRNA-378 and protein kinase Cα was not significant (r = 0.259, P = 0.232). Conclusions The expression of miRNA-328 and miRNA-378 in tumor tissues can be used to predict brain metastases in patients with non-small-cell lung cancer. miRNA-328 might promote brain metastases by regulating the expression of protein kinase Cα. However, the mechanisms of miRNA-378 to promote brain metastases should be studied in the future. |
| Databáze: | OpenAIRE |
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