Determinants of T box protein specificity
| Autor: | Elena S. Casey, Brenda M. J. Price, Frank L. Conlon, James C. Smith, Lynne Fairclough |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Fetal Proteins
Brachyury Embryo Nonmammalian Xenopus Molecular Sequence Data Eomesodermin Xenopus Proteins Biology Substrate Specificity Xbra Animals Point Mutation Amino Acid Sequence Binding site Molecular Biology Transcription factor Repetitive Sequences Nucleic Acid Genetics chemistry.chemical_classification Binding Sites Sequence Homology Amino Acid Lysine Gene Expression Regulation Developmental biology.organism_classification Amino acid T-box chemistry Female Asparagine T-Box Domain Proteins Developmental Biology |
| Zdroj: | Development. 128:3749-3758 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.128.19.3749 |
| Popis: | Members of the T box family of transcription factors play important roles in early development. Different members of the family exert different effects and here we show that much of the specificity of the Xenopus T box proteins Xbra, VegT and Eomesodermin resides in the DNA-binding domain, or T box. Binding site selection experiments show that the three proteins bind the same core sequence, but they select paired sites that differ in their orientation and spacing. Lysine 149 of Xbra is conserved in all Brachyury homologues, while the corresponding amino acid in VegT and Eomesodermin is asparagine. Mutation of this amino acid to lysine changes the inductive abilities of VegT and Eomesodermin to resemble that of Xbra. |
| Databáze: | OpenAIRE |
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