Effects on Capsicum annuum Plants Colonized with Trichoderma atroviride P. Karst Strains Genetically Modified in Taswo1, a Gene Coding for a Protein with Expansin-like Activity
| Autor: | Alfredo Herrera-Estrella, María del Rayo Sánchez-Carbente, Jorge Luis Folch-Mallol, Verónica Lira-Ruan, Carlos Alberto González-Chávez, Karina Atriztán-Hernández, Ayixon Sánchez-Reyes, Richa Mehta, Olivia Martínez-Villamil, Ricardo Sánchez-Cruz, Edgar Balcázar-López, Ramón Alberto Batista-García, María Luisa Tabche-Barrera, Roberto Carlos Bárcenas-Rodríguez |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Ecology
biology stress tolerance Inoculation SWOLLENIN Mutant Botany food and beverages phytopathogen resistance Plant Science biology.organism_classification Trichoderma Genetically modified organism Microbiology Cell wall Expansin QK1-989 Plant defense against herbivory Ecology Evolution Behavior and Systematics Systemic acquired resistance |
| Zdroj: | Plants, Vol 10, Iss 1919, p 1919 (2021) Plants Volume 10 Issue 9 |
| ISSN: | 2223-7747 |
| Popis: | Here, we analyzed the effects on Capsicum annuum plants of Trichoderma atroviride P. Karst strains altered in the expression of SWOLLENIN (SWO1), a protein with amorphogenic activity on plant cell wall components. Strains of T. atroviride that overexpressed the Taswo1 gene were constructed as well as deletion mutants. A novel, cheap and accurate method for assessing root colonization was developed. Colonization assays showed that the Taswo1 overexpressing strains invaded the host root better than the WT, resulting in a stronger plant growth-promoting effect. The expression of plant defense marker genes for both the systemic acquired resistance and induced systemic resistance pathways was enhanced in plants inoculated with Taswo1 overexpressing strains, while inoculation with deletion mutant strains resulted in a similar level of expression to that observed upon inoculation with the wild-type strain. Response to pathogen infection was also enhanced in the plants inoculated with the Taswo1 overexpressing strains, and surprisingly, an intermediate level of protection was achieved with the mutant strains. Tolerance to abiotic stresses was also higher in plants inoculated with the Taswo1 overexpressing strains but was similar in plants inoculated with the wild-type or the mutant strains. Compatible osmolyte production in drought conditions was studied. This study may contribute to improving Trichoderma biocontrol and biofertilization abilities. |
| Databáze: | OpenAIRE |
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