Genomic Analysis of Antimicrobial Resistance and Resistance Plasmids in Salmonella Serovars from Poultry in Nigeria
| Autor: | Vanesa García Menéndez, Iruka N. Okeke, Anders Dalsgaard, Abdurrahman Hassan Jibril, John Elmerdahl Olsen |
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| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía, School of Chemical and Biomedical Engineering |
| Rok vydání: | 2021 |
| Předmět: |
Bioengineering [Engineering]
0301 basic medicine Microbiology (medical) plasmids Salmonella Tetracycline 030106 microbiology Resistance Virulence Nigeria Biology medicine.disease_cause Biochemistry Microbiology Genome Poultry Salmonella genomic islands resistance 03 medical and health sciences Plasmid Antibiotic resistance Genomic island medicine Whole genome sequence Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics Genetics poultry lcsh:RM1-950 whole genome sequence lcsh:Therapeutics. Pharmacology 030104 developmental biology Infectious Diseases Mobile genetic elements medicine.drug Plasmids |
| Zdroj: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Jibril, A H, Okeke, I N, Dalsgaard, A, Menéndez, V G & Olsen, J E 2021, ' Genomic analysis of antimicrobial resistance and resistance plasmids in salmonella serovars from poultry in Nigeria ', Antibiotics, vol. 10, no. 2, 99, pp. 1-22 . https://doi.org/10.3390/antibiotics10020099 Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela Universidad de Santiago de Compostela (USC) Antibiotics Volume 10 Issue 2 Antibiotics, Vol 10, Iss 99, p 99 (2021) |
| DOI: | 10.3390/antibiotics10020099 |
| Popis: | Antimicrobial resistance is a global public health concern, and resistance genes in Salmonella, especially those located on mobile genetic elements, are part of the problem. This study used phenotypic and genomic methods to identify antimicrobial resistance and resistance genes, as well as the plasmids that bear them, in Salmonella isolates obtained from poultry in Nigeria. Seventy-four isolates were tested for susceptibility to eleven commonly used antimicrobials. Plasmid reconstruction and identification of resistance and virulence genes were performed with a draft genome using in silico approaches in parallel with plasmid extraction. Phenotypic resistance to ciprofloxacin (50.0%), gentamicin (48.6%), nalidixic acid (79.7%), sulphonamides (71.6%) and tetracycline (59.5%) was the most observed. Antibiotic resistance genes (ARGs) detected in genomes corresponded well with these observations. Commonly observed ARGs included sul1, sul2, sul3, tet (A), tet (M), qnrS1, qnrB19 and a variety of aminoglycoside-modifying genes, in addition to point mutations in the gyrA and parC genes. Multiple ARGs were predicted to be located on IncN and IncQ1 plasmids of S. Schwarzengrund and S. Muenster, and most qnrB19 genes were carried by Col (pHAD28) plasmids. Seventy-two percent (19/24) of S. Kentucky strains carried multidrug ARGs located in two distinct variants of Salmonella genomic island I. The majority of strains carried full SPI-1 and SPI-2 islands, suggesting full virulence potential This work was part-supported by an African Research Leader Award to INO from the UK Medical Research Council (MRC) and the UK Department for International Development (DFID) under the MRC/DFID Concordat agreement that is also part of the EDCTP2 programme supported by the European Union. VG acknowledges Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia for her post-doctoral grant (Grant Number ED481B-2018/018) SI |
| Databáze: | OpenAIRE |
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