In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
| Autor: | Frederico Ozanan Barros Monteiro, O. C. M. Pereira-Junior, J. F. Lima-Neto, Fernanda da Cruz Landim-Alvarenga, Sheila Canevese Rahal |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Federal Rural University of the Amazon Institute of Animal Health and Production |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Castor Oil
Bone Regeneration RD1-811 Surface Properties Cellular differentiation Polyurethanes lcsh:Surgery Bone Matrix Dogs Tissue engineering Osteogenesis Materials Testing medicine Cell Adhesion Animals Bone regeneration Cells Cultured Cell Proliferation Tissue Engineering Tissue Scaffolds Demineralized bone matrix Chemistry Stem Cells Mesenchymal stem cell Biomaterial Cell Differentiation Mesenchymal Stem Cells Anatomy lcsh:RD1-811 Cell biology medicine.anatomical_structure Durapatite Bone Substitutes Microscopy Electron Scanning Surgery Bone marrow Hydroxyapatites Stem cell |
| Zdroj: | Acta Cirurgica Brasileira, Vol 28, Iss 5, Pp 353-360 (2013) SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Acta Cirúrgica Brasileira v.28 n.5 2013 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Acta Cirurgica Brasileira, Volume: 28, Issue: 5, Pages: 353-360, Published: MAY 2013 |
| ISSN: | 0102-8650 |
| Popis: | Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:58:20Z No. of bitstreams: 1 S0102-86502013000500006.pdf: 2408747 bytes, checksum: df81830609f996bf6ef7748532357f1e (MD5) Made available in DSpace on 2013-08-22T18:58:20Z (GMT). No. of bitstreams: 1 S0102-86502013000500006.pdf: 2408747 bytes, checksum: df81830609f996bf6ef7748532357f1e (MD5) Previous issue date: 2013-05-01 Made available in DSpace on 2013-09-30T19:53:57Z (GMT). No. of bitstreams: 2 S0102-86502013000500006.pdf: 2408747 bytes, checksum: df81830609f996bf6ef7748532357f1e (MD5) S0102-86502013000500006.pdf.txt: 33527 bytes, checksum: 82cd1940ad21b4da2f5f4f8f724a2ad9 (MD5) Previous issue date: 2013-05-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:14:03Z No. of bitstreams: 2 S0102-86502013000500006.pdf: 2408747 bytes, checksum: df81830609f996bf6ef7748532357f1e (MD5) S0102-86502013000500006.pdf.txt: 33527 bytes, checksum: 82cd1940ad21b4da2f5f4f8f724a2ad9 (MD5) Made available in DSpace on 2014-05-20T15:14:03Z (GMT). No. of bitstreams: 2 S0102-86502013000500006.pdf: 2408747 bytes, checksum: df81830609f996bf6ef7748532357f1e (MD5) S0102-86502013000500006.pdf.txt: 33527 bytes, checksum: 82cd1940ad21b4da2f5f4f8f724a2ad9 (MD5) Previous issue date: 2013-05-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy. UNESP School of Veterinary Medicine and Animal Science Department of Veterinary Surgery and Anesthesiology UNESP School of Veterinary Medicine and Animal Department of Animal Reproduction and Veterinary Radiology Federal Rural University of the Amazon Institute of Animal Health and Production UNESP School of Veterinary Medicine and Animal Science Department of Veterinary Surgery and Anesthesiology UNESP School of Veterinary Medicine and Animal Department of Animal Reproduction and Veterinary Radiology |
| Databáze: | OpenAIRE |
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