Comparative Study of NAP-XPS and Cryo-XPS for the Investigation of Surface Chemistry of the Bacterial Cell-Envelope
| Autor: | Madeleine Ramstedt, Karin Schwibbert, Wolfgang E. S. Unger, Marit Kjaervik, Paul Dietrich |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Annan kemi
reference sample Atom and Molecular Physics and Optics Reference data (financial markets) Biophysics Pseudomonas fluorescens 02 engineering and technology 01 natural sciences Microbiology Bacterial cell structure X-ray photoelectron spectroscopy 0103 physical sciences hydrated samples bacteria QD1-999 Envelope (waves) Original Research DSM 50090 010304 chemical physics Strain (chemistry) Spectrometer biology Chemistry General Chemistry near ambient pressure XPS 021001 nanoscience & nanotechnology biology.organism_classification Biofysik Mikrobiologi C 1s spectra Chemical engineering Atom- och molekylfysik och optik cryo XPS 0210 nano-technology Other Chemistry Topics Ambient pressure |
| Zdroj: | Frontiers in Chemistry Frontiers in Chemistry, Vol 9 (2021) |
| Popis: | Bacteria generally interact with the environment via processes involving their cell-envelope. Thus, techniques that may shed light on their surface chemistry are attractive tools for providing an understanding of bacterial interactions. One of these tools is Al Kα-excited photoelectron spectroscopy (XPS) with its estimated information depth of Pseudomonas fluorescens using both techniques. We compare the results obtained and, in general, observe good agreement between the two techniques. Furthermore, we discuss advantages and disadvantages with the two analysis approaches and the output data they provide. XPS reference data from the bacterial strain are provided, and we propose that planktonic cells of this strain (DSM 50090) are used as a reference material for surface chemical analysis of bacterial systems. |
| Databáze: | OpenAIRE |
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