RNA synthesis by villus and crypt cell nuclei of rat intestinal mucosa
| Autor: | N.S. Leleiko, A.D. Bronstein, Hamish N. Munro |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
DNA Replication
Male Transcription Genetic Crypt Population Biophysics Cell Separation Biology digestive system Biochemistry chemistry.chemical_compound Intestinal mucosa Structural Biology Intestine Small Genetics medicine Animals Intestinal Mucosa education Cell Nucleus education.field_of_study digestive oral and skin physiology RNA Rats Inbred Strains Alkaline Phosphatase Rats Kinetics Cell nucleus medicine.anatomical_structure chemistry Thymidine kinase Alkaline phosphatase Thymidine |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 739:334-343 |
| ISSN: | 0167-4781 |
| Popis: | Rat intestinal mucosa was separated by eversion and vibration to provide a sequence of fractions from predominantly villus cells to predominantly crypt cells. The proportions of these cell types in each fraction were computed from the concentrations of alkaline phosphatase (villus cells) and thymidine kinase (crypt cells) in each population. The isolated mucosal fractions varied from about 90% villus cells to 90% crypt cells. Following injection of the rats with [3H]thymidine, the nuclei were isolated from each mucosal cell fraction and the amount of radioactivity incorporated into DNA was measured as an index of crypt cell abundance. The isolated nuclei were also incubated with ribonucleoside triphosphates and the amount of RNA synthesized was measured. Nuclei labeled with [3H]thymidine were found only in fractions rich in crypt cells, whereas capacity for RNA synthesis remained very active in mucosal fractions consisting predominantly of villus cells. It is concluded that non-dividing villus cells continue to make RNA. |
| Databáze: | OpenAIRE |
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