Gamma radiation effects on diamond field-effect biosensors with fibroblasts and extracellular matrix
| Autor: | Bohuslav Rezek, Jan Čermák, Marie Krátká, Jana Vachelová, Marie Davídková, Alexander Kromka, Nataliya Romanyuk |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Biocompatibility Scanning electron microscope Field effect 02 engineering and technology Biosensing Techniques Radiation engineering.material 01 natural sciences Colloid and Surface Chemistry 0103 physical sciences Miniaturization Physical and Theoretical Chemistry 010304 chemical physics business.industry Diamond Surfaces and Interfaces General Medicine Fibroblasts 021001 nanoscience & nanotechnology Fluorescence Extracellular Matrix engineering Microscopy Electron Scanning Optoelectronics 0210 nano-technology business Biosensor Biotechnology |
| Zdroj: | Colloids and surfaces. B, Biointerfaces. 204 |
| ISSN: | 1873-4367 |
| Popis: | Due to high biocompatibility, miniaturization, optical transparency and low production cost together with high radiation hardness the diamond-based sensors are considered promising for radiation medicine and biomedicine in general. Here we present detection of fibroblast cell culture properties by nanocrystalline diamond solution-gated field-effect transistors (SG-FET), including effects of gamma irradiation. We show that blank nanocrystalline diamond field-effect biosensors are stable at least up to 300 Gy of γ irradiation. On the other hand, gate current of the diamond SG-FET biosensors with fibroblastic cells increases exponentially over an order of magnitude with increasing radiation dose. Extracellular matrix (ECM) formation is also detected and analyzed by correlation of electronic sensor data with optical, atomic force, fluorescence, and scanning electron microscopies. |
| Databáze: | OpenAIRE |
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