Labbag® - a versatile bag-based cultivation system for expansion, differentiation and cryopreservation of human stem cells
| Autor: | Michael M. Gepp, Kristina Lachmann, Rowena Duckstein, Andrea Liebmann, Zuzana Scheuerer, Heiko Zimmermann, Julia C. Neubauer, Cornelia Stramm, Norbert Rodler, Michael Thomas, Fabian Kayatz |
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| Přispěvatelé: | Publica |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
organoid hanging droplet education Biomedical Engineering cell based model spheroids Biology 01 natural sciences Cryopreservation 010309 optics 03 medical and health sciences cell based models 0103 physical sciences Organoid human pluripotent stem cells hanging droplets organoids Spheroid Cell biology aggregate 030104 developmental biology spheroid aggregates Medicine Stem cell |
| Zdroj: | Current Directions in Biomedical Engineering, Vol 3, Iss 2, Pp 371-374 (2017) |
| ISSN: | 2364-5504 |
| DOI: | 10.1515/cdbme-2017-0077 |
| Popis: | Novel approaches in regenerative medicine and tissue engineering are highly promoted by human induced pluripotent stem cells. Typical workflows include the expansion, differentiation and/or cryopreservation of these cells. Often, the differentiation, cytotoxicity tests or disease modelling rely on multicellular aggregates. Here, we present the novel bag-based system “Labbag®” to address workflows required for these applications. As a unique feature, the Labbag® provides a chemical spot pattern to allow the formation of several hundreds of hanging droplets within a few seconds with standard laboratory equipment. In this study, we describe the general concept of the Labbag® and show aggregate formation of stem cells as first stem cell workflow successfully transferred. |
| Databáze: | OpenAIRE |
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