Resistance of glia‐like central and peripheral neural stem cells to genetically induced mitochondrial dysfunction—differential effects on neurogenesis
| Autor: | Ricardo Pardal, Verónica Sobrino, Blanca Diaz-Castro, José López-Barneo, José I. Piruat, Rocío Durán, Paula García-Flores |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Neurogenesis
Biochemistry Mice Neural Stem Cells Neurosphere Glial Fibrillary Acidic Protein Genetics medicine Animals Molecular Biology Neurons Carotid Body Glial fibrillary acidic protein biology Scientific Reports Brain Anatomy Neural stem cell Mitochondria Cell biology Succinate Dehydrogenase Neuroepithelial cell Disease Models Animal Oligodendroglia medicine.anatomical_structure nervous system Astrocytes biology.protein Neuroglia Stem cell Gene Deletion Adult stem cell |
| Zdroj: | EMBO reports. 16:1511-1519 |
| ISSN: | 1469-3178 1469-221X |
| Popis: | Mitochondria play a central role in stem cell homeostasis. Reversible switching between aerobic and anaerobic metabolism is critical for stem cell quiescence, multipotency, and differentiation, as well as for cell reprogramming. However, the effect of mitochondrial dysfunction on neural stem cell (NSC) function is unstudied. We have generated an animal model with homozygous deletion of the succinate dehydrogenase subunit D gene restricted to cells of glial fibrillary acidic protein lineage (hGFAP-SDHD mouse). Genetic mitochondrial damage did not alter the generation, maintenance, or multipotency of glia-like central NSCs. However, differentiation to neurons and oligodendrocytes (but not to astrocytes) was impaired and, hence, hGFAP-SDHD mice showed extensive brain atrophy. Peripheral neuronal populations were normal in hGFAP-SDHD mice, thus highlighting their non-glial (non hGFAP(+)) lineage. An exception to this was the carotid body, an arterial chemoreceptor organ atrophied in hGFAP-SDHD mice. The carotid body contains glia-like adult stem cells, which, as for brain NSCs, are resistant to genetic mitochondrial damage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text