Search of Neuroprotective Polyphenols Using the 'Overlay' Isolation Method
| Autor: | Kenjiro Bandow, Misaki Horiuchi, Hiroshi Sakagami, Akito Tomomura, Haixia Shi, Takaaki Oizumi, Mineko Tomomura, Tomohiro Fujisawa |
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| Rok vydání: | 2018 |
| Předmět: |
Cell Culture Techniques
Pharmaceutical Science Epigallocatechin gallate Resveratrol Pharmacology PC12 Cells Catechin Culture Media Serum-Free overlay method Analytical Chemistry chemistry.chemical_compound 0302 clinical medicine Blood serum hormesis Nerve Growth Factor Stilbenes Drug Discovery bamboo leaf extract Cytotoxic T cell Neurons NGF Cell Differentiation PC12 differentiation taxanes Neuroprotective Agents Chemistry (miscellaneous) 030220 oncology & carcinogenesis Molecular Medicine Taxoids neuroprotection Sasa Curcumin amyloid-β peptide Neuroprotection Article lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry Animals Physical and Theoretical Chemistry Amyloid beta-Peptides Plant Extracts Organic Chemistry Polyphenols Rats Plant Leaves polyphenol Nerve growth factor chemistry Polyphenol 030217 neurology & neurosurgery |
| Zdroj: | Molecules, Vol 23, Iss 8, p 1840 (2018) Molecules Volume 23 Issue 8 Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry |
| ISSN: | 1420-3049 |
| Popis: | Previous studies of the neuroprotective activity of polyphenols have used ununiform culture systems, making it difficult to compare their neuroprotective potency. We have established a new and simple method for preparing differentiated PC12 cells by removing the toxic coating step. Cells were induced to differentiate with the nerve growth factor (NGF) in a serum-free medium, without a medium change, but with a one-time overlay supplementation of NGF. The optimal inoculation density of the cells was 6&ndash 12 × 103 cells/cm2, and the presence of serum inhibited the differentiation. Neuroprotective activity could be quantified by the specific index (SI) value, that is, the ratio of the 50% cytotoxic concentration to the 50% effective concentration. Alkaline extract from the leaves of Sasa senanensis Rehder (SE), having had hormetic growth stimulation, showed the highest SI value, followed by epigallocatechin gallate. The SI value of curcumin and resveratrol was much lower. This simple overly method, that can prepare massive differentiated neuronal cells, may be applicable for the study of the differentiation-associated changes in intracellular metabolites, and the interaction between neuronal cells and physiological factors. |
| Databáze: | OpenAIRE |
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