Type IV pili promote early biofilm formation by Clostridium difficile
| Autor: | Grace A. Maldarelli, Robert K. Ernst, Alison J. Scott, Yang Song, Erik C. von Rosenvinge, Yvonne Achermann, Jeffrey A. Freiberg, Kurt H. Piepenbrink, Mark E. Shirtliff, Eric J. Sundberg, Michael S. Donnenberg |
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| Přispěvatelé: | University of Zurich, von Rosenvinge, Erik C |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Mutant Mutagenesis (molecular biology technique) 610 Medicine & health Biology Pilus 2726 Microbiology (medical) Microbiology 10234 Clinic for Infectious Diseases 03 medical and health sciences 2400 General Immunology and Microbiology Immunology and Allergy Cluster Analysis Humans Enterocolitis Pseudomembranous General Immunology and Microbiology Clostridioides difficile Gene Expression Profiling Biofilm RNA High-Throughput Nucleotide Sequencing General Medicine Gene Expression Regulation Bacterial 2725 Infectious Diseases Clostridium difficile biochemical phenomena metabolism and nutrition Complementation 030104 developmental biology Infectious Diseases Phenotype Pilin Biofilms Fimbriae Bacterial Mutation biology.protein 2723 Immunology and Allergy Fimbriae Proteins Transcriptome Research Article |
| Zdroj: | Pathogens and Disease Pathogens and disease |
| DOI: | 10.1093/femspd/ftw061 |
| Popis: | Increasing morbidity and mortality from Clostridium difficile infection (CDI) present an enormous challenge to healthcare systems. Clostridium difficile express type IV pili (T4P), but their function remains unclear. Many chronic and recurrent bacterial infections result from biofilms, surface-associated bacterial communities embedded in an extracellular matrix. CDI may be biofilm mediated; T4P are important for biofilm formation in a number of organisms. We evaluate the role of T4P in C. difficile biofilm formation using RNA sequencing, mutagenesis and complementation of the gene encoding the major pilin pilA1, and microscopy. RNA sequencing demonstrates that, in comparison to other growth phenotypes, C. difficile growing in a biofilm has a distinct RNA expression profile, with significant differences in T4P gene expression. Microscopy of T4P-expressing and T4P-deficient strains suggests that T4P play an important role in early biofilm formation. A non-piliated pilA1 mutant forms an initial biofilm of significantly reduced mass and thickness in comparison to the wild type. Complementation of the pilA1 mutant strain leads to formation of a biofilm which resembles the wild-type biofilm. These findings suggest that T4P play an important role in early biofilm formation. Novel strategies for confronting biofilm infections are emerging; our data suggest that similar strategies should be investigated in CDI. |
| Databáze: | OpenAIRE |
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