Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis
| Autor: | Katiany Rizzieri Caleffi-Ferracioli, Pedro Henrique Canezin, João Vítor Perez de Souza, Rosilene Fressatti Cardoso, Letícia Sayuri Murase, Vera Lúcia Dias Siqueira, Fernando Rogério Pavan, Regiane Bertin de Lima Scodro |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Tuberculosis Time Factors Genotype 030106 microbiology Antitubercular Agents Microbial Sensitivity Tests Microbiology Mycobacterium tuberculosis 03 medical and health sciences Minimum inhibitory concentration Bacterial Proteins Drug Resistance Multiple Bacterial Tuberculosis Multidrug-Resistant medicine Humans biology Membrane Transport Proteins Drug Synergism DNA-Directed RNA Polymerases Gene Expression Regulation Bacterial biology.organism_classification medicine.disease Catalase 030104 developmental biology Real-time polymerase chain reaction Verapamil Mutation Drug Therapy Combination Efflux Rifampin Oxidoreductases Rifampicin Brazil medicine.drug |
| Zdroj: | Future microbiology. 14 |
| ISSN: | 1746-0921 |
| Popis: | Aim: To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in Mycobacterium tuberculosis. Materials & methods: RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs. Results: VP showed good combinatory effect among RIF-resistant isolates. This effect was also observed in the relative transcript levels of EPs, mainly after 72 h of exposure, depending on the EP gene, genotype and the resistance profile of the isolate. Conclusion: Additional regulatory mechanisms in the EP activities, as well as, interactions with other drug-specific resistance mechanisms need further investigation in M. tuberculosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|