Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies
| Autor: | Julia Stein, Bárbara Campos Jorge, Ana Carolina Casali Reis, Joyce Alencar Santos Radai, Suyane da Silva Moreira, Thiago Leite Fraga, Jonas da Silva Mota, Rodrigo Juliano Oliveira, Cândida Aparecida Leite Kassuya, Arielle Cristina Arena |
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| Přispěvatelé: | Universidade Estadual Paulista (UNESP), UNIGRAN, Universidade Estadual de Mato Grosso do Sul (UEMS), Universidade Federal de Mato Grosso do Sul (UFMS), UFGD |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Dose-Response Relationship
Drug Mutagenicity Tests Plant Extracts Hepatotoxicity General Medicine Piperaceae Toxicology Rats Plant Leaves Random Allocation Mice Blood Toxicity Tests Subacute Liver Piper amalago Animals Female Rats Wistar Genotoxicity Safety Subacute toxicity Piper Blood Chemical Analysis Hemato-biochemical alterations DNA Damage |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2022-05-01T12:40:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Piper amalago L. (Piperaceae) is traditionally used due to its anti-inflammatory, analgesic, diuretic, and antiparasitic properties. However, few studies have focused on its adverse effects, compromising its safe use. This study evaluated the toxicological safety of ethanolic extract from Piper amalago leaves (EEPA), through subacute toxicity and genotoxicity assays in rodents. In subacute toxicity, 100, 200 or 300 mg/kg of EEPA were tested in female Wistar rats, by gavage, for 28 days. For genotoxicity test, female Swiss mice were orally treated with 17.5, 175 or 1750 mg/kg of EEPA and the comet, micronucleus, and splenic phagocytic assays were evaluated. In subacute toxicity, the extract induced an increase in the food and water intakes, as well as in the liver absolute weight, and in the heart and kidney relative weights. EEPA also provoked alterations in histopathological analysis of liver and in hemato-biochemical parameters, evidenced by a decrease in hematocrit levels and albumin levels, and an increase in the number of platelets and in alkaline phosphatase and cholesterol levels. However, EEPA did not presented genotoxic nor mutagenic properties. EEPA showed hemato-biochemical toxicity profile in rats and should be used with caution, especially when for prolonged period. Department of Structural and Functional Biology Institute of Biosciences of Botucatu UNESP – Univ. Estadual Paulista - Botucatu, São Paulo State School of Health Sciences University Center of Grande Dourados UNIGRAN Mato Grosso do Sul State University UEMS Federal University of Mato Grosso do Sul UFMS School of Health Sciences Federal University of Grande Dourados UFGD Department of Structural and Functional Biology Institute of Biosciences of Botucatu UNESP – Univ. Estadual Paulista - Botucatu, São Paulo State CNPq: #304266/2017–5 CNPq: #311166/2016–4 CNPq: #427859/2018–2 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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