Development and validation of a method for human papillomavirus genotyping based on molecular beacon probes
Autor: | Yu Yuefeng, Ren Xuyi, Pan Caixia, Jiangfeng Lyu, Jing Zhou |
---|---|
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Viral Diseases Genotyping Techniques Artificial Gene Amplification and Extension Pathology and Laboratory Medicine Cervical Cancer Polymerase Chain Reaction law.invention Human Papillomavirus DNA Tests law Genotype Medicine and Health Sciences DNA Probes HPV Papillomaviridae Polymerase chain reaction Cervical cancer Multidisciplinary Hybridization probe Physics Melting Condensed Matter Physics Infectious Diseases Molecular Diagnostic Techniques Oncology Medical Microbiology Viral Pathogens Viruses Physical Sciences Medicine Pathogens Phase Transitions Research Article Human Papillomavirus Infection Genotyping Papillomaviruses HPV-6 Science Urology 030106 microbiology Sexually Transmitted Diseases Molecular Probe Techniques Biology Research and Analysis Methods Sensitivity and Specificity Microbiology Melting curve analysis 03 medical and health sciences Molecular beacon medicine Humans Human papillomavirus Molecular Biology Techniques Microbial Pathogens Molecular Biology Biology and life sciences Genitourinary Infections Papillomavirus Infections Organisms Human Papillomavirus Cancers and Neoplasms medicine.disease Virology Probe Hybridization DNA viruses Gynecological Tumors |
Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 11, p e0207930 (2018) |
ISSN: | 1932-6203 |
Popis: | We describe a new assaying system for the detection and genotyping of human papillomavirus (HPV) based on linear-after-the-exponential-PCR(LATE-PCR) and melting curve analysis. The 23 most prevalent HPV strains (types 6, 11, 16, 18, 31, 33, 35, 39, 42, 45, 51, 52, 53, 56, 58, 59, 66, 68, 70, 73, 81, 82, and 83) are assayed in two sealed reaction tubes within 2 h. Good sensitivity and specificity was evaluated by testing cloned HPV DNA and clinical samples. The detection limit was 5-500 copies/reaction depending on the genotype. No cross-reactivity was observed with the other HPV types that are not covered by our method or pathogens tested which were commonly found in female genital tract. When compared with the HPV GenoArray Diagnostic kit, the results from 1104 clinical samples suggest good overall agreement between the two methods,(98.37%, 95% CI: 97.44%-98.97%) and the kappa value was 0.954. Overall, this new HPV genotyping assay system presents a simple, rapid, universally applicable, sensitive, and highly specific detection methodology that should be useful for HPV detection and genotyping, therefore, is potentially of great value in clinical application. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |